The mouse kinome: Discovery and comparative genomics of all mouse protein kinases
The mouse kinome: Discovery and comparative genomics of all mouse protein kinases
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DOI：
10.1073/pnas.0306880101
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发表时间：
2004-08-10
影响因子：
11.1
通讯作者：
Manning, G
中科院分区：
文献类型：
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作者：
Caenepeel, S;Charydczak, G;Manning, G
我们已经确定了小鼠的全部蛋白激酶（PK）补体（激酶组）。这组540个基因包含许多新的激酶，以及对150多个已发表序列的修正或扩展。小鼠拥有518种人类蛋白激酶中的510种的直系同源物。非直系同源激酶仅通过反转录转座和基因衰退产生。直系同源激酶对在其全长的序列保守性上存在差异，为每一对激酶绘制了功能重要区域的图谱。存在许多物种特异性的序列插入片段，并且它们经常发生可变剪接，从而产生进化谱系特异性的功能。发现了97个激酶假基因，它们都不同于107个人类激酶假基因。染色体定位将163种激酶与突变表型相关联，并开启了利用小鼠遗传学来确定直系同源人类激酶功能的途径。
We have determined the full protein kinase (PK) complement (kinome) of mouse. This set of 540 genes includes many novel kinases and corrections or extensions to >150 published sequences. The mouse has orthologs for 510 of the 518 human PIKs. Nonorthologous kinases arise only by retrotransposition and gene decay. Orthologous kinase pairs vary in sequence conservation along their length, creating a map of functionally important regions for every kinase pair. Many species-specific sequence inserts exist and are frequently alternatively spliced, allowing for the creation of evolutionary lineage-specific functions. Ninety-seven kinase pseudogenes were found, all distinct from the 107 human kinase pseudogenes. Chromosomal mapping links 163 kinases to mutant phenotypes and unlocks the use of mouse genetics to determine functions of orthologous human kinases.