The mannose receptor mediates uptake of soluble but not of cell-associated antigen for cross-presentation
The mannose receptor mediates uptake of soluble but not of cell-associated antigen for cross-presentation
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DOI：
10.4049/jimmunol.176.11.6770
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发表时间：
2006-06-01
影响因子：
4.4
通讯作者：
Kurts, Christian
中科院分区：
文献类型：
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作者：
Burgdorf, Sven;Lukacs-Kornek, Veronika;Kurts, Christian
甘露糖受体（MR）参与微生物和血清糖蛋白的识别和清除。它的内吞功能已经使用巨噬细胞进行了广泛的研究，尽管它由多种细胞类型表达，包括树突状细胞（DC）。在这项研究中，我们研究了其在DC使用MR-/-小鼠的Ag呈递中的作用。吸收的模型银，可溶性卵清蛋白，骨髓来源的DC和体外激活的卵清蛋白特异性的CD 8 T细胞（OT-I细胞）严格依赖于MR。在体内，MR缺乏受损的可溶性卵清蛋白的DC和伴随OT-I细胞激活的内吞作用。MR-/-小鼠的DC亚型组成没有改变。细胞相关的OVA的摄取不受MR缺陷的影响，导致OT-I细胞的活化不变。这些发现表明，DC使用MR进行旨在交叉呈递的特定Ag类型的内吞。
The mannose receptor (MR) has been implicated in the recognition and clearance of microorganisms and serum glycoproteins. Its endocytic function has been studied extensively using macrophages, although it is expressed by a variety of cell types, including dendritic cells (DC). In this study, we investigated its role in Ag presentation by DC using MR-/- mice. Uptake of the model Ag, soluble OVA, by bone marrow-derived DC and in vitro activation of OVA-specific CD8 T cells (OT-I cells) strictly depended on the MR. In vivo, MR deficiency impaired endocytosis of soluble OVA by DC and concomitant OT-I cell activation. No alterations in the DC subtype composition in MR-/- mice were accountable. Uptake of cell-associated OVA was unaffected by MR deficiency, resulting in unchanged activation of OT-I cells. These findings demonstrate that DC use the MR for endocytosis of a particular Ag type intended for cross-presentation.