Angiotensin II and phorbol ester enhance isoproterenol- and vasoactive intestinal peptide (VIP)-induced cyclic AMP accumulation in vascular smooth muscle cells.
Angiotensin II and phorbol ester enhance isoproterenol- and vasoactive intestinal peptide (VIP)-induced cyclic AMP accumulation in vascular smooth muscle cells.
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DOI：
10.1016/0006-291x(85)91765-6
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发表时间：
1985-08
影响因子：
3.1
通讯作者：
T. Nabika;Y. Nara;Y. Yamori;W. Lovenberg;J. Endo
中科院分区：
文献类型：
--
作者：
T. Nabika;Y. Nara;Y. Yamori;W. Lovenberg;J. Endo
Ca2+和cAMP在细胞功能调节中的重要性已经得到很好的证明。本实验观察了血管紧张素II（AII）对异丙肾上腺素（ISO）和血管活性肠肽（VIP）诱导的血管平滑肌细胞（VSMC）cAMP蓄积的影响。虽然单独添加AII几乎没有引起cAMP的增加，但它以剂量依赖性方式增强ISO和VIP诱导的cAMP积累。这种增强是模仿肿瘤促进佛波酯，但不是由Ca2+离子载体。这一观察结果表明，AII增强激动剂诱导的cAMP的积累，通过激活蛋白激酶C在VSMC。
The importance of Ca2+and cAMP in the regulation of cellular functions has been well demonstrated. We studied the effect of angiotensin II (AII), a potent Ca2+-mobilizing hormone, on cAMP accumulation induced by isoproterenol (ISO) and vasoactive intestinal peptide (VIP) in cultured vascular smooth muscle cells (VSMC). Although the addition of AII alone caused little increase of cAMP, it enhanced ISO- and VIP-induced cAMP accumulations in a dose-dependent manner. This enhancement was mimicked by tumor-promoting phorbol ester but not by Ca2+ionophore. This observation suggested that AII enhanced agonist-induced cAMP accumulation through the activation of protein kinase C in VSMC.