Leukocytes in a Plasmodium falciparum-infected blood meal reduce transmission of malaria to Anopheles mosquitoes
Leukocytes in a Plasmodium falciparum-infected blood meal reduce transmission of malaria to Anopheles mosquitoes
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DOI：
10.1128/iai.65.9.3834-3837.1997
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发表时间：
1997-09-01
影响因子：
3.1
通讯作者：
Sauerwein, RW
中科院分区：
文献类型：
--
作者：
Lensen, AHW;BolmervandeVegte, M;Sauerwein, RW
蚊子通过从配子体携带者身上吸食血液而感染恶性疟原虫。由于一只蚊子的体积为1至2 μ l，一顿血粉可能含有1 × 10（4）至3 × 10（4）个白细胞（WBC）。白细胞主要由嗜中性粒细胞组成，嗜中性粒细胞可以吞噬和杀死蚊子中肠内发育中的配子。在恶性疟原虫配子的存在下，与对照相比，来自地方性地区的血清具有增加的CL反应。在蚊子膜喂养实验中，一些这样的血清显示出与活WBC的存在相关的传播减少。本研究的结果表明，在蚊子中肠内发生调理配子的吞噬作用，并且可以有助于减少恶性疟原虫寄生虫的传播。
Mosquitoes are infected with Plasmodium falciparum by taking a blood meal from a gametocyte carrier. Since a mosquito takes a volume of 1 to 2 mu l, a blood meal may contain 1 x 10(4) to 3 x 10(4) leukocytes (WBC). The majority of WBC are composed of neutrophils which may phagocytose and kill developing gametes inside the mosquito midgut, Phagocytosis was measured in vitro by a luminol-dependent chemiluminescence (CL) assay. In the presence of P. falciparum gametes, sera from areas of endemicity had an increased CL response compared to controls, In mosquito membrane feeding experiments some such sera showed a transmission reduction which was related to the presence of viable WBC, The results of this study suggest that phagocytosis of opsonized gametes inside the mosquito midgut occurs and can contribute to a reduction in the transmission of P. falciparum parasites.