Molecular characterisation of the vitellogenin gene (AlVg) and its expression after Apolygus lucorum had fed on different hosts
Molecular characterisation of the vitellogenin gene (AlVg) and its expression after Apolygus lucorum had fed on different hosts
复制标题
卵黄原基因（AlVg）的分子特征及其在不同寄主取食后的表达
DOI：
10.1002/ps.4203
复制
发表时间：
2016
影响因子：
4.1
通讯作者：
Bai Lixin
中科院分区：
文献类型：
--
作者：
Sun Yang;Xiao Liubin;Cao Guangchun;Zhang Yongjun;Xiao Yingfang;Xu Guangchun;Zhao Jing;Tan Yongan;Bai Lixin
摘要绿盲蝽是我国Bt棉的主要害虫。本研究通过分析不同宿主对AlVg基因转录和翻译的影响，探讨AlVg基因和蛋白表达与A.不同的寄主营养物质能显著调节拟南芥TSAlVgor AlVg的表达（P< 0.05）。AlVg或AlVg在A组中的表达显著高于B组。B和常规棉的光合速率比A高。在加兰菊花和蚕豆上培养的苜蓿中，AlVg和AlVg的表达量差异不显著（P> 0.05）。而普通棉与Bt棉相比差异不显著（P> 0.05）。此外，AlVg或AlVg的表达量与雌虫总死亡率、雌虫寿命、单雌产卵量和卵孵化率之间均存在显著的线性回归关系（P<0. 05）。AlVg和AlVg的表达除受Bt毒素影响外，还受不同寄主营养物质的影响。© 2015化学工业协会
BACKGROUNDThe polyphagous pestApolygus lucorumis now the dominant pest ofBacillus thuringiensis(Bt) cotton in China. In this study, the transcriptional and translational profiles of AlVg influenced by different hosts were identified, and then the correlations betweenAlVggene or AlVg protein expression and key population proliferation parameters ofA. lucorumwere further clarified.RESULTSAlVgor AlVg expression can be significantly regulated by different host nutrients (P< 0.05). AlVg orAlVgexpression was significantly higher inA. lucorumreared onBtand conventional cotton than inA. lucorumreared on garland chrysanthemum and broad bean (P< 0.05), but there was no significant difference between AlVg orAlVgexpression inA. lucorumreared onBtand conventional cotton (P> 0.05). In addition, there were significant linear regression correlations betweenAlVgor AlVg expression and total mortality rate of nymphs, female lifespan, per female fecundity and egg hatching rates (P< 0.05).CONCLUSIONOur results confirm thatAlVgor AlVg is the key parameter affecting female fertility ofA. lucorum.AlVgand AlVg expression can be influenced by different host nutrients except forBttoxin. © 2015 Society of Chemical Industry