Organic cation transporter 3 (OCT3) is localized to intracellular and surface membranes in select glial and neuronal cells within the basolateral amygdaloid complex of both rats and mice.
Organic cation transporter 3 (OCT3) is localized to intracellular and surface membranes in select glial and neuronal cells within the basolateral amygdaloid complex of both rats and mice.
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DOI：
10.1007/s00429-016-1315-9
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发表时间：
2017-05
影响因子：
3.1
通讯作者：
Pickel VM
中科院分区：
文献类型：
--
作者：
Gasser PJ;Hurley MM;Chan J;Pickel VM
有机阳离子转运蛋白 3 (OCT3) 是一种高容量、低亲和力转运蛋白，介导皮质酮敏感的单胺摄取，包括去甲肾上腺素、肾上腺素、多巴胺、组胺和血清素。 OCT3 在杏仁复合体和其他大脑区域中广泛表达，其中单胺是受压力影响的情绪行为的关键调节剂。然而，评估 OCT3 对单胺能神经传递调节和单胺依赖性行为调节的贡献需要有关 OCT3 表达的亚细胞分布的基本信息。我们使用免疫荧光和免疫电子显微镜检查大鼠和小鼠大脑基底外侧杏仁复合体中转运蛋白的细胞和亚细胞分布。在大鼠和小鼠杏仁核的胶质细胞和神经元周核中均观察到 OCT3 免疫反应性。电子显微镜免疫标记揭示了与各种突触相邻的轴突、树突和星形细胞过程以及神经元胞体上质膜相关的 OCT3 免疫反应性。除了质膜位点外，还观察到 OCT3 免疫标记与神经元和神经胶质内膜（包括高尔基体、线粒体和核膜）相关。在神经元、星形细胞、小胶质细胞和内皮细胞核周中观察到外核膜的特别显着的标记。 OCT3 定位于邻近突触位点的神经元和神经胶质质膜，这与该转运蛋白在调节释放的单胺的幅度、持续时间和物理扩散方面的重要作用一致，而其定位于线粒体和外核膜表明该转运蛋白在单胺的细胞内处置中具有先前未描述的作用。
Organic cation transporter 3 (OCT3) is a high-capacity, low-affinity transporter that mediates corticosterone-sensitive uptake of monoamines including norepinephrine, epinephrine, dopamine, histamine and serotonin. OCT3 is expressed widely throughout the amygdaloid complex and other brain regions where monoamines are key regulators of emotional behaviors affected by stress. However, assessing the contribution of OCT3 to the regulation of monoaminergic neurotransmission and monoamine-dependent regulation of behavior requires fundamental information about the subcellular distribution of OCT3 expression. We used immunofluorescence and immuno-electron microscopy to examine the cellular and subcellular distribution of the transporter in the basolateral amygdaloid complex of the rat and mouse brain. OCT3-immunoreactivity was observed in both glial and neuronal perikarya in both rat and mouse amygdala. Electron microscopic immunolabeling revealed plasma membrane-associated OCT3 immunoreactivity on axonal, dendritic, and astrocytic processes adjacent to a variety of synapses, as well as on neuronal somata. In addition to plasma membrane sites, OCT3 immunolabeling was also observed associated with neuronal and glial endomembranes, including Golgi, mitochondrial and nuclear membranes. Particularly prominent labeling of the outer nuclear membrane was observed in neuronal, astrocytic, microglial and endothelial perikarya. The localization of OCT3 to neuronal and glial plasma membranes adjacent to synaptic sites is consistent with an important role for this transporter in regulating the amplitude, duration, and physical spread of released monoamines, while its localization to mitochondrial and outer nuclear membranes suggests previously undescribed roles for the transporter in the intracellular disposition of monoamines.