Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis
Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis
复制标题
DOI：
10.1002/ijc.23016
复制
发表时间：
2007-12-15
影响因子：
6.4
通讯作者：
Zhao, Fu-Kun
中科院分区：
文献类型：
--
作者：
Lin, Jian-Feng;Xu, Jun;Zhao, Fu-Kun
虽然血清前列腺特异性抗原（PSA）是一种公认的前列腺癌（PCa）检测的诊断工具，但前列腺癌的最终诊断是基于前列腺穿刺活检（PNBX）标本中包含的信息。为了确定PNBX标本的蛋白质组学特征以确定PCa的候选生物标志物，我们对来自PCa或良性前列腺增生（BPH）患者的PNBX标本进行了比较蛋白质组学分析。2-DE结果显示，在PCa组和BPH组中，52个蛋白斑点的变化具有统计学意义。利用MALDI-TOF-MS/MS鉴定感兴趣点。鉴定出的两组最值得注意的蛋白质包括潜在雄激素受体共调节因子[FLNA（7-15）和FKBP4]和参与线粒体脂肪酸p -氧化的酶（DCI和ECHS1）。在PCa标本中发现了过氧化物还氧蛋白亚型表达的不平衡。此外，还鉴定了HSP27和HSP70.1不同的翻译后修饰亚型。重要的是，FLNA（7-15）、FKBP4和PRDX4表达的变化通过免疫印迹分析得到证实。我们的研究结果表明，基于蛋白质组学的方法有助于开发更完整的PNBX标本蛋白质图谱。通过这种方法鉴定的蛋白质可能是前列腺癌诊断和治疗的有用分子靶点。(c) 2007 Wiley-Liss, Inc。
Although serum prostate specific antigen (PSA) is a well-established diagnostic tool for prostate cancer (PCa) detection, the definitive diagnosis of PCa is based on the information contained in prostate needle biopsy (PNBX) specimens. To define the proteomic features of PNBX specimens to identify candidate biomarkers for PCa, PNBX specimens from patients with PCa or benign prostatic hyperplasia (BPH) were subjected to comparative proteomic analysis. 2-DE revealed that 52 protein spots exhibited statistically significantly changes among PCa and BPH groups. Interesting spots were identified by MALDI-TOF-MS/MS. The 2 most notable groups of proteins identified included latent androgen receptor coregulators [FLNA(7-15) and FKBP4] and enzymes involved in mitochondrial fatty acid P-oxidation (DCI and ECHS1). An imbalance in the expression of peroxiredoxin subtypes was noted in PCa specimens. Furthermore, different post-translationally modified isoforms of HSP27 and HSP70.1 were identified. Importantly, changes in FLNA(7-15), FKBP4, and PRDX4 expression were confirmed by immunoblot analyses. Our results suggest that a proteomics-based approach is useful for developing a more complete picture of the protein profile of PNBX specimen. The proteins identified by this approach may be useful molecular targets for PCa diagnostics and therapeutics. (c) 2007 Wiley-Liss, Inc.