EST-SSR sequences revealed the relationship of D-genome in diploid and tetraploid Species in Gossypium
EST-SSR sequences revealed the relationship of D-genome in diploid and tetraploid Species in Gossypium
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DOI：
10.1016/j.plantsci.2008.12.007
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发表时间：
2009-03-01
期刊：
影响因子：
5.2
通讯作者：
Guo, Wang-Zhen
中科院分区：
文献类型：
--
作者：
Zhu, Hua-Yu;Zhang, Tian-Zhen;Guo, Wang-Zhen
四倍体棉花包括两个亚基因组，分别命名为A亚基因组和D亚基因组。在这项研究之前，四倍体棉花物种中的D亚基因组供体仍然难以捉摸。在以前的研究中，简单重复序列是由G. raimondii在G.棉和海岛棉[W.Z. Guo，C.P. Cai，C.B. Wang，Z.G. Han，X.L.宋，K.王晓文纽角，澳-地Wang，K.Y. Lu，B. Shi，T.Z. Zhang，A microsatellite-based，gene-rich linkage map reveals genome structure，function，and evolution in Gossypium，Genetics 176（2007）527-541].我们质疑D基因组棉花物种在四倍体棉花进化中的作用。本研究利用14对EST-SSR引物对G. raimondii对棉属23个种进行了扩增。总共对438个扩增子进行了克隆、测序和分析。利用SSR侧翼序列构建了13个D基因组二倍体物种和8个AD基因组四倍体物种的系统发育树。结果表明，13个D基因组物种符合Fryxell的亚分类。D亚基因组四倍体物种与D基因组二倍体物种的亲缘关系表明，G。raimondii是所有四倍体物种中唯一的D基因组供体。(c)2008爱思唯尔爱尔兰有限公司保留所有权利。
Tetraploid cotton species included two subgenomes, named as A-subgenome and D-subgenome, respectively. Prior to this study, the D-subgenome donor in tetraploid cotton species remained elusive. In a previous study, simple sequence repeats developed from expressed sequence tags (EST-SSRs) of G. raimondii was found to possess a high polymorphic frequency between G. hirsutum and G. barbadense [W.Z. Guo, C.P. Cai, C.B. Wang, Z.G. Han, X.L. Song, K. Wang, X.W. Niu, C. Wang, K.Y. Lu, B. Shi,T.Z. Zhang, A microsatellite-based, gene-rich linkage map reveals genome structure, function, and evolution in Gossypium, Genetics 176 (2007) 527-541]. We questioned the role of the D-genome cotton species in the evolution of tetraploid cotton. In this paper, 14 EST-SSR primer pairs from G. raimondii were used to amplify 23 species in Gossypium. In total, 438 amplicons were cloned, sequenced and analyzed. A phylogenetic tree of 13 D-genome diploid species and 8 AD-genome tetraploid species was constructed based on the combined simple sequence repeat (SSR) flanking sequence data. The results showed that 13 D-genome species were congruent with Fryxell's subsection taxonomy. The relationship between D-subgenome tetraploid species and D-genome diploid species indicated that G. raimondii is the sole D-genome donor of all tetraploid species. (c) 2008 Elsevier Ireland Ltd. All rights reserved.