Quantification of Myocardial Perfusion Defect Size in Rats: Comparison between Quantitative Perfusion SPECT and Autoradiography
Quantification of Myocardial Perfusion Defect Size in Rats: Comparison between Quantitative Perfusion SPECT and Autoradiography
复制标题
大鼠心肌灌注缺损大小的量化：定量灌注 SPECT 与放射自显影的比较
DOI：
10.1007/s11307-018-1159-1
复制
发表时间：
2018
期刊：
影响因子：
3.1
通讯作者：
Kinuya S
中科院分区：
文献类型：
--
作者：
Wakabayashi H;Taki J;Inaki A;Hiromasa T;Okuda K;Shibutani T;Shiba K;Kinuya S
目的将多针孔准直器的超高分辨率单光子发射计算机断层扫描（SPECT）系统应用于小型啮齿动物的成像。我们旨在比较定量灌注单光子发射计算机断层扫描（QPS; cedar - sinai Medical Center， USA）与高分辨率放射自显像在大鼠模型上的心肌梗死（MI）面积，以确定QPS测量灌注缺陷的准确性。9只大鼠开胸后左冠状动脉闭塞再灌注，注射185 MBq [99mTc]甲氧基异丁基异腈（[99mTc]MIBI）进行SPECT和放射自显像。对健康大鼠（n= 28）进行类似扫描，以建立一个正常的数据库，并以此为基础进行QPS。用QPS软件自动分析SPECT图像上的心肌梗死区域。对于放射自显像图像，MI感兴趣的区域被设置为1mm间隔。结果正常大鼠[99mTc]MIBI在整个左心室积聚，心室灌注极谱图显示下壁（68%±4%）和前壁（92%±5%）摄取最低和最高。在大鼠心肌梗死模型中，mbi摄取减少[99mTc]的极图百分比与放射自显像显示的左心室心肌梗死百分比密切相关（r2= 0.90）。结论sqps可以定量评价大鼠心肌灌注图像的心肌梗死严重程度，与放射自显像结果相当。这种广泛使用的软件可以促进分析小动物心脏图像的新技术的发展。
PurposeUltra-high resolution single-photon emission computed tomography (SPECT) system, using multiple pinhole collimators, has been applied to the imaging of small rodents. We aimed to compare the myocardial infarction (MI) area on quantitative perfusion single-photon emission computed tomography (QPS; Cedars-Sinai Medical Center, USA) with that on high-resolution autoradiography in rat model to determine the accuracy of perfusion defect measurement by QPS.ProceduresAfter thoracotomy, rats (n= 9) had their left coronary arteries occluded and reperfused before injection with 185 MBq [99mTc] methoxyisobutylisonitrile ([99mTc]MIBI) for SPECT and autoradiography. Healthy rats (n= 28) were similarly scanned to create a normal database on which to base QPS. The MI area on SPECT images was analysed automatically by QPS software. For the autoradiography images, regions of interest for MI were set at 1 mm intervals.ResultsIn normal rats, [99mTc]MIBI accumulated throughout the left ventricles, and a polar map of ventricular perfusion showed the lowest and highest uptakes in the inferior (68 % ± 4 %) and anterior (92 % ± 5 %) walls, respectively. In the rat MI model, the percentage of polar map with reduced [99mTc]MIBI uptake correlated strongly with the percentage of left ventricle with MI on autoradiography (r2= 0.90).ConclusionsQPS can quantitatively evaluate MI severity on myocardial perfusion images in rats, with comparable results to autoradiography. This widely available software could promote the development of new techniques for analysing cardiac images in small animals.