Establishment of human cell lines showing circadian rhythms of bioluminescence
Establishment of human cell lines showing circadian rhythms of bioluminescence
复制标题
显示生物发光昼夜节律的人类细胞系的建立
DOI：
10.1016/j.neulet.2008.08.091
复制
发表时间：
2008
影响因子：
2.5
通讯作者：
T. Ebisawa
中科院分区：
文献类型：
--
作者：
Aki Yoshikawa;Hiroko Shimada;K. Numazawa;Tsukasa Sasaki;M. Ikeda;Minae Kawashima;Nobumasa Kato;K. Tokunaga;T. Ebisawa
我们已经建立了稳定表达荧光素酶基因的人视网膜色素上皮细胞系，由人Bmal1启动子驱动，以获得地塞米松治疗后显示生物发光昼夜节律的人源性细胞。获得的克隆的平均生物发光昼夜周期为24.07±0.48h。培养基中的锂（10 mM）显着延长了生物发光的昼夜节律周期，这与以前的报道一致，而2 mM或5 mM锂没有影响。这是关于建立无限增殖并显示生物发光昼夜节律的人源细胞系的第一份报告，也是第一份研究低剂量锂对体外人源细胞昼夜节律影响的报告。所建立的细胞将可用于人类昼夜节律的各种体外研究，以及用于开发与昼夜节律紊乱相关的人类疾病的新疗法。
We have established human retinal pigment epithelial cell lines stably expressing the luciferase gene, driven by the human Bmal1 promoter, to obtain human-derived cells that show circadian rhythms of bioluminescence after dexamethasone treatment. The average circadian period of bioluminescence for the obtained clones was 24.07±0.48h. Lithium (10mM) in the medium significantly lengthened the circadian period of bioluminescence, which is consistent with previous reports, while 2mM or 5mM lithium had no effect. This is the first report on the establishment of human-derived cell lines that proliferate infinitely and show circadian rhythms of bioluminescence, and also the first to investigate the effects of low-dose lithium on the circadian rhythms of human-derived cells in vitro. The established cells will be useful for various in vitro studies of human circadian rhythms and for the development of new therapies for human disorders related to circadian rhythm disturbances.
影响因子：
2.7
作者：
Leclerc, GM;Boockfor, FR;Frawley, LS
通讯作者：
Frawley, LS