Doxorubicin-encapsulated thermosensitive liposome-functionalized photothermal composite scaffolds for synergistic photothermal therapy and chemotherapy
Doxorubicin-encapsulated thermosensitive liposome-functionalized photothermal composite scaffolds for synergistic photothermal therapy and chemotherapy
复制标题
阿霉素封装的热敏脂质体功能化光热复合支架用于协同光热治疗和化疗
DOI：
10.1039/d2tb00993e
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发表时间：
2022
影响因子：
7
通讯作者：
Yang Yingnan,Chen Guoping
中科院分区：
文献类型：
--
作者：
Chen Huajian;Sun Rui;Zheng Jing;Kawazoe Naoki;Yang Yingnan,Chen Guoping
协同疗法，特别是光热疗法和化疗的结合，已被提出作为乳腺癌治疗的有效治疗方法。在本研究中，通过将阿霉素封装的热敏脂质体和金纳米棒杂交到明胶和聚谷氨酸的多孔支架（Dox-lipo/AuNR/Gel/PGA）中，开发了协同光热疗法和化疗的智能平台。 Dox-lipo/AuNR/Gel/PGA复合支架具有良好的光热转化和温度依赖性阿霉素释放特性。在近红外激光照射下，复合支架提高了局部温度，不仅杀死了支架内的乳腺癌细胞，而且加速了阿霉素的释放，消除了支架周围的乳腺癌细胞。体外细胞培养和体内小鼠实验表明，在近红外激光照射下，光热消融与阿霉素诱导的复合支架内及其周围乳腺癌细胞的抑制相结合具有协同效应。此外，药物释放完成后，复合支架促进人骨髓源性间充质干细胞增殖。这些结果表明，复合支架为早期乳腺癌细胞消除提供协同光热疗法和化疗，并在晚期促进干细胞活性。因此，这种复合支架作为乳腺癌治疗的协同治疗平台具有巨大的潜力。
Synergistic therapy, especially the combination of photothermal therapy and chemotherapy, has been proposed as an effective therapeutic approach for breast cancer treatment. In this study, a smart platform for synergistic photothermal therapy and chemotherapy was developed by hybridizing doxorubicin-encapsulated thermosensitive liposomes and gold nanorods into porous scaffolds of gelatin and polyglutamic acid (Dox-lipo/AuNR/Gel/PGA). The Dox-lipo/AuNR/Gel/PGA composite scaffolds had good photothermal conversion and temperature-dependent doxorubicin release properties. Under near-infrared laser irradiation, the composite scaffolds increased the local temperature to not only kill the breast cancer cells in the scaffolds but also accelerate the release of doxorubicin to eliminate the breast cancer cells surrounding the scaffolds. In vitro cell culture and in vivo mouse experiments demonstrated that the synergistic effects of photothermal ablation combined with doxorubicin-induced inhibition of the breast cancer cells in and surrounding the composite scaffolds under near-infrared laser irradiation. Moreover, after drug release was complete, the composite scaffolds fostered human bone marrow-derived mesenchymal stem cell proliferation. These results suggested that the composite scaffolds provided synergistic photothermal therapy and chemotherapy for breast cancer cell elimination at the early stage and promoted stem cell activities at the late stage. Therefore, this composite scaffold holds great potential as a synergistic therapy platform for breast cancer treatment.