Investigating the impact of condomless vaginal intercourse and lubricant use on the vaginal metabolome: a pre-post observational study.
Investigating the impact of condomless vaginal intercourse and lubricant use on the vaginal metabolome: a pre-post observational study.
复制标题
调查无套阴道性交和润滑剂使用对阴道代谢组的影响：一项前后观察研究。
DOI：
10.1136/sextrans-2022-055667
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发表时间：
2023
影响因子：
3.6
通讯作者：
Yeoman,CarlJ
中科院分区：
文献类型：
--
作者：
Borgogna,Joanna-LynnC;Grace,SavannahG;Holm,JohannaB;AvilesZuniga,Tadeo;Kadriu,Herlin;He,Xin;McCoski,SarahR;Ravel,Jacques;Brotman,RebeccaM;Yeoman,CarlJ
阴道代谢组是阴道微环境中的一个重要因素，数据显示其在泌尿生殖健康中的独立作用。无避孕套阴道性交和个人润滑剂的使用是可能影响阴道代谢组的常见做法。本研究的目的是描述之间的关联避孕套性交和润滑剂的使用对阴道metabolome.MethodsThis研究使用存档中期阴道拭子从一个10周的观察队列的育龄妇女谁自我收集的样本和记录的行为日记每天。病例组和对照组被定义为分别自我报告使用或不使用润滑剂的无避孕套阴道性交的参与者。在无避孕套阴道性交前后采集样本。22例病例参与者与22例对照参与者的种族/民族匹配。对阴道中段拭子进行16 S rRNA基因扩增子测序和非靶向高效液相色谱串联质谱代谢组学。贝叶斯混合效应回归（未调整和调整的阴道微生物群）被用来评估与阴道性交和润滑剂use.ResultsBoth避孕套阴茎阴道性交和润滑剂的使用代谢物浓度的差异独立与较高的（高达8.3倍）浓度的代谢物指示上皮损伤（如，肌氨酸）和许多主机产生的抗氧化剂。在一项控制性交独立效应的研究设计中，润滑剂的使用与细胞损伤相关的脂质增加、宿主产生的鞘脂（抗菌剂）、抗氧化剂和水杨酸盐（润滑剂常见的冷却剂）显著相关。代谢参与氧化应激和水杨酸盐强烈相关的几个分子细菌阴道病相关bacteries.ConclusionsThis研究提供了重要的基础数据如何避孕套阴道阴茎性交和润滑剂的使用影响阴道代谢组，并可能影响在阴道微环境的保护机制。
ObjectiveThe vaginal metabolome is a significant factor in the vaginal microenvironment, and data are emerging on its independent role in urogenital health. Condomless vaginal intercourse and personal lubricant use are common practices that may affect the vaginal metabolome. The aim of the present study is to describe the associations between condomless intercourse and lubricant use on the vaginal metabolome.MethodsThis study used archived mid-vaginal swabs from a 10-week observational cohort of reproductive age women who self-collected samples and recorded behavioural diaries daily. Cases and controls were defined as participants who self-reported condomless vaginal intercourse with or without lubricant use, respectively. Samples were drawn prior to and following condomless vaginal intercourse. Twenty-two case participants were race/ethnicity matched to 22 control participants. Mid-vaginal swabs were subjected to 16S rRNA gene amplicon sequencing and untargeted ultrahigh performance liquid chromatography tandem mass spectroscopy metabolomics. Bayesian mixed-effects regression (unadjusted and adjusted for the vaginal microbiota) was used to evaluate differences in metabolite concentration associated with vaginal intercourse and lubricant use.ResultsBoth condomless penile–vaginal intercourse and lubricant use were independently associated with higher (up to 8.3-fold) concentrations of metabolites indicative of epithelial damage (eg, sarcosine) and many host-produced antioxidants. Lubricant use was significantly associated with increases in lipids related to cellular damage, host-produced sphingolipids (antimicrobials), antioxidants and salicylate, a cooling agent common to lubricants, in a study design which controls for the independent effect of intercourse. Metabolites involved in oxidative stress and salicylate were strongly correlated with several molecular bacterial vaginosis-associated bacteria.ConclusionsThis study provides important foundational data on how condomless vaginal–penile intercourse and lubricant use affect the vaginal metabolome and may affect the protective mechanisms in the vaginal microenvironment.