Modulation of Foreign Body Reaction against PDMS Implant by Grafting Topographically Different Poly(acrylic acid) Micropatterns
Modulation of Foreign Body Reaction against PDMS Implant by Grafting Topographically Different Poly(acrylic acid) Micropatterns
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DOI：
10.1002/mabi.201900206
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发表时间：
2019-11-10
影响因子：
4.6
通讯作者：
Koh, Won-Gun
中科院分区：
文献类型：
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作者：
Lee, Jae Sang;Shin, Byung Ho;Koh, Won-Gun
聚二甲基硅氧烷（PDMS）的表面通过表面引发的光聚合与聚丙烯酸（PAA）层接枝，以抑制由异物反应引起的包囊挛缩。由于光致聚合的性质，可以使用光刻法制造各种PAA微图案。将宽约100 μ m、厚约3 μ m的孔和条纹微图案无分层地接枝到PDMS表面上。PAA微图案的掺入不仅通过亲水性PAA微域提供化学线索，而且通过孔或条纹微图案提供形貌线索。体外研究表明，PAA接枝的PDMS表面具有较低的巨噬细胞（Raw 264.7）和成纤维细胞（NIH 3T3）增殖，而不管模式的存在。然而，具有PAA微图案，特别是条纹微图案的PDMS使成纤维细胞的聚集及其随后分化成肌成纤维细胞最小化。体内研究还表明，具有条纹微图案的PDMS样品将巨噬细胞极化为抗炎M2巨噬细胞，并且最有效地抑制包囊挛缩，这通过研究炎症评分、转化生长因子β表达、巨噬细胞和肌成纤维细胞的数量以及胶原密度和包囊厚度来证明。
The surface of poly(dimethylsiloxane) (PDMS) is grafted with poly(acrylic acid) (PAA) layers via surface-initiated photopolymerization to suppress the capsular contracture resulting from a foreign body reaction. Owing to the nature of photo-induced polymerization, various PAA micropatterns can be fabricated using photolithography. Hole and stripe micropatterns approximate to 100-mu m wide and 3-mu m thick are grafted onto the PDMS surface without delamination. The incorporation of PAA micropatterns provides not only chemical cues by hydrophilic PAA microdomains but also topographical cues by hole or stripe micropatterns. In vitro studies reveal that a PAA-grafted PDMS surface has a lower proliferation of both macrophages (Raw 264.7) and fibroblasts (NIH 3T3) regardless of the pattern presence. However, PDMS with PAA micropatterns, especially stripe micropatterns, minimizes the aggregation of fibroblasts and their subsequent differentiation into myofibroblasts. An in vivo study also shows that PDMS samples with stripe micropatterns polarized macrophages into anti-inflammatory M2 macrophages and most effectively inhibits capsular contracture, which is demonstrated by investigation of inflammation score, transforming-growth-factor-beta expression, number of macrophages, and myofibroblasts as well as the collagen density and capsule thickness.