PHENYLISOTHIOCYANATE DERIVATIZATION OF N-TAU-METHYLHISTIDINE - A METHOD OF ASSESSING MYOFIBRILLAR PROTEIN-DEGRADATION
PHENYLISOTHIOCYANATE DERIVATIZATION OF N-TAU-METHYLHISTIDINE - A METHOD OF ASSESSING MYOFIBRILLAR PROTEIN-DEGRADATION
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DOI：
10.1016/s0271-5317(89)80149-6
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发表时间：
1989-11-01
影响因子：
4.5
通讯作者：
LIU, CC
中科院分区：
文献类型：
--
作者：
FORSBERG, NE;LIU, CC
尿N-甲基组氨酸（NMH）排泄已被用作肌原纤维蛋白降解（MPD）的指标。本文介绍了一种测定生长期不同甲状腺状态大鼠尿NMH水平的方法。从对照、甲状腺切除（Tx）和补充三碘甲状腺原氨酸的甲状腺切除大鼠（T3）中采集尿液样本，用于开发该方法。尿NMH和标准NMH经脱乙酰基后，用异硫氰酸苯酯（PITC）衍生化，进样至高效液相色谱（HPLC）系统，在254 nm处检测。尿液中的峰在保留时间上与标准品PITC-NMH一致。该峰的质谱证明其由分子量等于PITC-NMH的分子量的化合物组成。本研究中大鼠排泄的NMH的量与其他研究中报告的NMH排泄模式相似。甲状腺切除术，减少MPD，减少尿NMH水平，而T3管理，这已被报道，以提高MPD，增加尿NMH排泄。因此，异硫氰酸苯酯衍生化提供了一种快速和敏感的方法来评估NMH排泄模式在生长大鼠。
Urinary NT-methylhistidine (NMH) excretion has been used as an index of myofibrillar protein degradation (MPD). This paper describes a method for assessment of urinary NMH levels in growing rats of variable thyroid status. Samples urine were collected from control, thyroidectomized (Tx), and triiodothyronine-supplemented thyroidectomized rats (T3) for development of this method. Urinary NMH and standard NMH were deacetylated then derivatized with phenylisothiocyanate (PITC) and injected into a high-performance liquid chromatography (HPLC) system and detected at 254 nm. A peak in urine corresponded in retention time to standard PITC-NMH. Mass spectroscopy of this peak demonstrated that it consisted of a compound of molecular mass equal to that of PITC-NMH. Quantities of NMH excreted by rats in this study were similar to NMH excretion patterns reported in other studies. Thyroidectomy, which reduces MPD, reduced urinary NMH levels whereas T3 administration, which has been reported to enhance MPD, increased urinary NMH excretion. Phenylisothiocyanate derivatization, therefore, provides a rapid and sensitive method for assessing NMH excretion patterns in growing rats.