CNPase Expression in Olfactory Ensheathing Cells
CNPase Expression in Olfactory Ensheathing Cells
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DOI：
10.1155/2011/608496
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发表时间：
2011-01-01
影响因子：
--
通讯作者：
Kocsis, Jeffery D.
中科院分区：
文献类型：
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作者：
Radtke, Christine;Sasaki, Masanori;Kocsis, Jeffery D.
大量的研究支持嗅觉鞘细胞（OECs）移植到神经或脊髓损伤中可以促进轴突再生和髓鞘再生的建议。然而，一些研究人员质疑移植的oec是否与轴突结合并形成外周髓磷脂，或者它们是否招募内源性雪旺细胞形成髓磷脂。研究了在2-3-环核苷酸3-磷酸二酯酶启动子控制下表达增强型绿色荧光蛋白（eGFP）的转基因小鼠嗅球。CNPase在髓磷脂形成细胞的整个谱系中表达。我们检测了嗅球原位和体外CNPase的表达，以确定oec是否表达与其髓鞘形成潜力相称的CNPase。在嗅球外神经层可见eGFP。体外培养的游离OECs具有强烈的eGFP表达和CNPase免疫染色。oec移植到与再生轴突纵向相关的横断周围神经。这些数据表明，CNPase转基因小鼠嗅球外神经层的oec表达CNPase。因此，虽然oec通常不会在嗅觉神经轴突上形成髓磷脂，但当它们移植到受损的周围神经中时，CNPase的表达与其形成髓磷脂的潜力是相称的。
A large body of work supports the proposal that transplantation of olfactory ensheathing cells (OECs) into nerve or spinal cord injuries can promote axonal regeneration and remyelination. Yet, some investigators have questioned whether the transplanted OECs associate with axons and form peripheral myelin, or if they recruit endogenous Schwann cells that form myelin. Olfactory bulbs from transgenic mice expressing the enhanced green fluorescent protein (eGFP) under the control of the 2-3-cyclic nucleotide 3-phosphodiesterase (CNPase) promoter were studied. CNPase is expressed in myelin-forming cells throughout their lineage. We examined CNPase expression in both in situ in the olfactory bulb and in vitro to determine if OECs express CNPase commensurate with theirmyelination potential. eGFP was observed in the outer nerve layer of the olfactory bulb. Dissociated OECs maintained in culture had both intense eGFP expression and CNPase immunostaining. Transplantation of OECs into transected peripheral nerve longitudinally associated with the regenerated axons. These data indicate that OECs in the outer nerve layer of the olfactory bulb of CNPase transgenic mice express CNPase. Thus, while OECs do not normally form myelin on olfactory nerve axons, their expression of CNPase is commensurate with their potential to form myelin when transplanted into injured peripheral nerve.