In vivo detection of c-Met expression in a rat C6 glioma model.
In vivo detection of c-Met expression in a rat C6 glioma model.
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DOI：
10.1111/j.1582-4934.2008.00220.x
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发表时间：
2008-01
影响因子：
5.3
通讯作者：
Lupu F
中科院分区：
文献类型：
--
作者：
Towner RA;Smith N;Doblas S;Tesiram Y;Garteiser P;Saunders D;Cranford R;Silasi-Mansat R;Herlea O;Ivanciu L;Wu D;Lupu F
The tyrosine kinase receptor, c-Met, and its substrate, the hepatocyte growth factor (HGF), are implicated in the malignant progression of glioblastomas. In vivo detection of c-Met expression may be helpful in the diagnosis of malignant tumours. The C6 rat glioma model is a widely used intracranial brain tumour model used to study gliomas experimentally. We used a magnetic resonance imaging (MRI) molecular targeting agent to specifically tag the cell surface receptor, c-Met, with an anti-c-Met antibody (Ab) linked to biotinylated Gd (gadolinium)-DTPA (diethylene triamine penta acetic acid)-albumin in rat gliomas to detect overexpression of this antigen in vivo. The anti-c-Met probe (anti-c-Met-Gd-DTPA-albumin) was administered intravenously, and as determined by an increase in MRI signal intensity and a corresponding decrease in regional T1 relaxation values, this probe was found to detect increased expression of c-Met protein levels in C6 gliomas. In addition, specificity for the binding of the anti-c-Met contrast agent was determined by using fluorescence microscopic imaging of the biotinylated portion of the targeting agent within neoplastic and ‘normal’brain tissues following in vivo administration of the anti-c-Met probe. Controls with no Ab or with a normal rat IgG attached to the contrast agent component indicated no non-specific binding to glioma tissue. This is the first successful visualization of in vivo overexpression of c-Met in gliomas.
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影响因子：
3.3
作者：
Israely, T;Dafni, H;Neeman, M
通讯作者：
Neeman, M
DOI：
10.1093/jnci/91.18.1548
发表时间：
1999-09-15
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Abounader, R;Ranganathan, S;Laterra, J
通讯作者：
Laterra, J
影响因子：
10.3
作者：
Legler, JM;Ries, LAG;Linet, MS
通讯作者：
Linet, MS
影响因子：
2.9
作者：
Dafni, H;Landsman, L;Neeman, M
通讯作者：
Neeman, M
影响因子：
11.5
作者：
Athauda, Gagani;Giubellino, Alessio;Bottaro, Donald P.
通讯作者：
Bottaro, Donald P.