Adult Donor Rod Photoreceptors Integrate into the Mature Mouse Retina
Adult Donor Rod Photoreceptors Integrate into the Mature Mouse Retina
复制标题
DOI：
10.1167/iovs.10-6329
复制
发表时间：
2011-07-01
影响因子：
4.4
通讯作者：
Reh, Thomas A.
中科院分区：
文献类型：
--
作者：
Gust, Juliane;Reh, Thomas A.
目的。以往的研究表明，出生后早期的小鼠光感受器在移植后能够整合到成熟的宿主视网膜中，而祖细胞和完全分化的光感受器则不能。作者试图确定随着年龄的增长，移植后光感受器整合能力的下降是否与成年神经元迁移能力的丧失或存活率的下降有关。方法：将分离的视网膜细胞从绿色荧光蛋白阳性(GFP(+))供体小鼠移植到年龄从胚胎12.5天到成年(出生后28天[P])的小鼠。免疫荧光检测标志物的表达和整合细胞的形态。分离的NRL-GFP小鼠的体外培养用于检测存活率。结果：先前的报道证实，新生杆状细胞整合到成年宿主中。然而，与以前的报道相反，供体细胞的年龄并不像以前报道的那样关键，因为研究发现，P11以上的供体细胞有效地整合到成年宿主视网膜中。尽管成年光感受器(P28及以上)比未成熟光感受器(P5)表现出更高的移植失败率，但对于成熟和未成熟供者来说，成功的移植具有非常相似的整合细胞数量。所有年龄的整合细胞在形态和标记表达上与宿主的细胞没有区别。结论：完全成熟的光感受器保留了整合到成熟视网膜的能力。作者认为，它们的整合潜力主要受到它们解离后存活率下降的限制。(投资眼科VS科学。2011年；52：5266-5272)doi：10.1167/iovs.10-6329
PURPOSE. Previous studies indicate that early postnatal mouse photoreceptors have the ability to integrate into the mature host retina after transplantation, while progenitors and fully differentiated photoreceptors do not. The authors sought to determine whether the decline in the ability of photoreceptors to integrate after transplantation with increasing age is related to a loss of migratory ability in the adult neurons or by a decrease in their survival.METHODS. Dissociated retinal cells were transferred from green fluorescent protein-positive (GFP(+)) donor mice of ages ranging from embryonic day (E) 12.5 to adults (>28 days postnatal [P]). Immunofluorescence was used to assess marker expression and the morphology of integrated cells. In vitro cultures of dissociated Nrl-GFP mice were used to assay survival.RESULTS. It was confirmed in previous reports that neonatal rods integrate into adult hosts. However, in contrast to previous reports, the age of the donor cell was not as critical as previously reported, because it was found that donor cells older than P11 effectively integrated into adult host retina. Although fully adult photoreceptors (P28 and older) show a higher transplant failure rate than immature ones (P5), successful transplants had very similar numbers of integrated cells for both mature and immature donors. Integrated cells from all ages were indistinguishable from those of the host in morphology and marker expression.CONCLUSIONS. Fully mature photoreceptors retain the ability to integrate into the mature retina. The authors propose that their potential for integration is limited primarily by their decreased survival after dissociation. (Invest Ophthalmol Vis Sci. 2011; 52:5266-5272) DOI: 10.1167/iovs.10-6329