Adsorption of microorganisms onto an artificial siderophore-modified Au substrate
Adsorption of microorganisms onto an artificial siderophore-modified Au substrate
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DOI：
10.1016/j.bios.2007.08.015
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发表时间：
2007-12-15
影响因子：
12.6
通讯作者：
Masuda, Hideki
中科院分区：
文献类型：
--
作者：
Inomata, Tornohiko;Eguchi, Hiroshi;Masuda, Hideki
制备了羟基酸盐型人工铁载体三[2-{3-（n -乙酰基- n -羟氨基）丙基}丙基]氨基甲烷（TAPPA）及其Fe-III配合物Fe-III-TAPPA，并用多种光谱方法对其进行了表征。Fe-III-TAPPA对黄微杆菌（Microbacterium flavescens）表现出生物活性，表明Fe-III-TAPPA可以渗透微生物的细胞膜。fe - iii -铁载体配合物通过逐步自组装的方法吸附在沉积的Au衬底上。Fe-III-TAPPA/Au电极的循环伏安图证实了Fe-III-TAPPA在表面的修饰作用。采用光学显微镜、扫描电子显微镜、原子力显微镜和石英晶体微天平（QCM）测量了铁- iii - tappa /Au对黄芽孢杆菌的吸附实验。这些结果清楚地表明，Fe-III-TAPPA/Au具有固定黄芽孢杆菌的作用。这种吸附特性是由于Au电极上的Fe-III-TAPPA与细胞膜内的受体/结合蛋白之间的相互作用。(c) 2007 Elsevier B.V.版权所有
The hydroxamate-type artificial siderophore, tris[2-{3-(N-acetyl-N-hydroxamino)propylamido}propyl]aminomethane (TAPPA) and its Fe-III complex, Fe-III-TAPPA were prepared and characterized by several spectroscopic methods. Fe-III-TAPPA exhibits biological activity for the hydroxamate-type siderophore auxotrophic microorganism, Microbacterium flavescens, suggesting that Fe-III-TAPPA can permeate the cell membrane of the microorganism. The adsorption of the Fe-III-siderophore complex onto a deposited Au substrate was achieved by a stepwise self-assembling method. The modification of Fe-III-TAPPA on the surface was confirmed from the cyclic voltammogram of the resultant Au electrode, Fe-III-TAPPA/Au. The adsorption experiments of M.flavescens with Fe-III-TAPPA/Au were monitored by optical, scanning electron, and atomic force microscopy and quartz crystal microbalance (QCM) measurements. These results clearly indicate that Fe-III-TAPPA/Au can immobilize M.flavescens. This adsorption characteristic is due to the interaction between Fe-III-TAPPA on an Au electrode and a receptor/binding protein within the cell membrane. (c) 2007 Elsevier B.V. All rights reserved.