The Chlamlydophila abortus genome sequence reveals an array of variable proteins that contribute to interspecies variation
The Chlamlydophila abortus genome sequence reveals an array of variable proteins that contribute to interspecies variation
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DOI：
10.1101/gr.3684805
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发表时间：
2005-05-01
期刊：
影响因子：
7
通讯作者：
Longbottom, D
中科院分区：
文献类型：
--
作者：
Thomson, NR;Yeats, C;Longbottom, D
原鹦鹉热衣原体流产亚型的专性胞内致病菌流产衣原体S26/3是反刍动物和猪妊娠晚期流产的重要原因。此外，虽然相对罕见，但人畜共患感染会导致急性疾病和孕妇流产。与其他衣原科植物相比，该植物在序列和总基因含量上具有较高的保守性。该基因组共1144377 bp，包含961个预测编码序列，其中842个与caviae衣原体和肺炎衣原体保守。在这个保守的Cp. abortus核心基因中，我们已经确定了主要的变异区域，并将我们的分析重点集中在这些位点上，其中几个位点被发现编码高度可变的蛋白质家族，如TMH/Inc和Pmp家族，这些家族是该生物群体的多样性来源，宿主趋向性和疾病病因的强有力候选者。值得注意的是，Cp. abortus缺乏任何毒素基因，也缺乏参与色氨酸代谢和核粒回收的基因（guaB作为假基因存在），这表明该物种的生态位适应的遗传基础与之前提出的其他衣原体物种不同。
The obligate intracellular bacterial pathogen Chlamydophila abortus strain S26/3 (formerly the abortion Subtype of Chlamydia psittaci) is all important Cause of late gestation abortions ill ruminants and pigs. Furthermore, although relatively rare, zoonotic infection call result ill acute illness and miscarriage ill pregnant women. The complete genome sequence was determined and shows a high level of conservation in both sequence and overall gene content in comparison to other Chlamydiaceae. The 1,144,377-bp genome contains 961 predicted coding sequences, 842 of which are conserved with those of Chlamydophila caviae and Chlamydophila pneumoniae. Within this conserved Cp. abortus core genorne we have identified the major regions of variation and have focused our analysis oil these loci, several of which were found to encode highly variable protein families, such as TMH/Inc and Pmp families, which are strong candidates for the Source of diversity ill host tropism and disease causation ill this group of organisms. Significantly, Cp. abortus lacks any toxin genes, and also lacks genes involved in tryptophan metabolism and nUcleoticle salvaging (guaB is present as a psuedogene), suggesting that the genetic basis of niche adaptation of this species is distinct from those previously proposed for other chlamyclial species.