Expression profiles of various transporters for oligopeptides, amino acids and organic ions along the human digestive tract.
Expression profiles of various transporters for oligopeptides, amino acids and organic ions along the human digestive tract.
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DOI：
10.1016/j.bcp.2005.09.027
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发表时间：
2005-12
影响因子：
5.8
通讯作者：
T. Terada;Y. Shimada;Xiaoyue Pan;K. Kishimoto;T. Sakurai;R. Doi;H. Onodera;T. Katsura;M. Imamura;K. Inui
中科院分区：
文献类型：
--
作者：
T. Terada;Y. Shimada;Xiaoyue Pan;K. Kishimoto;T. Sakurai;R. Doi;H. Onodera;T. Katsura;M. Imamura;K. Inui
各种转运蛋白如H+/肽协同转运蛋白PEPT 1在肠道中表达，并在体内发挥重要的生理和药理作用。本研究旨在检测20种转运蛋白（PEPT 1和2、P-糖蛋白、氨基酸转运蛋白和有机离子转运蛋白）在人消化道沿着的表达谱，重点研究了PEPT 1的表达谱。使用正常粘膜标本，进行实时聚合酶链反应。还对PEPT 1表达进行免疫印迹分析。与其他组织相比，PEPT 1 mRNA在小肠（十二指肠>空肠>回肠）中高度表达，一些患者在胃中显示出显著水平的表达。PEPT 1在胃组织中的表达模式和组织学诊断表明，胃PEPT 1起源于肠上皮化生。氨基酸转运蛋白在消化道中表现出独特的mRNA表达水平和分布。例如，从十二指肠到回肠，Na+依赖性和氯离子非依赖性中性氨基酸转运蛋白B 0AT 1的表达水平增加，其模式与PEPT 1完全相反。除了有机阳离子/肉毒碱转运蛋白OCTN 2外，几乎没有有机离子转运蛋白的表达。结论：PEPT 1在小肠中表达丰富，与B 0AT 1的相互表达可能有助于蛋白质消化产物的高效吸收。
Various transporters such as H+/peptide cotransporter PEPT1 are expressed in the intestine, and play important physiological and pharmacological roles in the body. Present study was performed to examine the expression profile of 20 kinds of transporters (PEPT1 and 2, P-glycoprotein, amino acid transporters and organic ion transporters) along the human digestive tract, especially focusing on PEPT1. Using normal mucosal specimens, real-time polymerase chain reactions were carried out. Immunoblot analyses were also performed for PEPT1 expression. PEPT1 mRNA was highly expressed in the small intestine (duodenum>jejunum>ileum) compared to other tissues, and some patients showed a significant level of expression in the stomach. The expressional pattern of PEPT1 in the stomach and histological diagnosis indicated that gastric PEPT1 originated from the intestinal metaplasia. The amino acid transporters showed unique mRNA expression levels and distributions in the digestive tract. For example, the expression levels of B0AT1, a Na+-dependent and chloride-independent neutral amino acid transporter, were increased from the duodenum to ileum, which pattern is completely inverted to that for PEPT1. There is little expression of organic ion transporters except for organic cation/carnitine transporter OCTN2. In conclusion, PEPT1 was abundantly expressed in the small intestine, and the reciprocal expression of PEPT1 and B0AT1 may serve for the efficient absorption of protein digestive products.