Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery
Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery
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DOI：
10.3791/55214
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发表时间：
2017-02-01
影响因子：
1.2
通讯作者：
Taffet, George E.
中科院分区：
文献类型：
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作者：
Granillo, Alejandro;Pena, Celia A.;Taffet, George E.
目前的方法教导研究者如何通过超声心动图测量和使用LAV作为左室舒张压慢性升高的替代品，以及获得小鼠主动脉和PA直径的测量。10日龄以上的小鼠可以使用本技术进行分析。该技术由3个主要步骤组成：设置、图像采集和图像分析。设置步骤包括将小鼠用1%异氟醚麻醉，剃须，并将其平躺在加热的心电图板上，在那里将进行图像采集。图像获取步骤包括学习识别心脏结构，并获得所有所需的图像及其相应的探头和轴，以便能够计算体积和直径。图像分析步骤是在计算机软件的帮助下对先前获取的图像进行测量。所提出的技术的优点包括快速（15分钟）的程序，使研究人员能够以非侵入性、非终末期的方法评估干预措施，因此可以长期跟踪同一只小鼠；每个鼠标都可以作为自己的控制。这一事实加上由同一操作员执行整个实验的所有采集和分析，最大限度地减少了操作员依赖性的限制。本方法对心血管和肺部医学的小鼠研究人员有用。
The present methodology teaches the investigator how to measure and use the LAV as a surrogate of chronic elevations in Left Ventricular diastolic pressure through echocardiography, as well as to obtain measurements of the Aorta and PA diameter in mice.Mice older than 10 d of age can be analyzed using the present technique. The technique is composed of 3 main steps: set-up, image acquisition, and image analysis. The set-up step consists of getting the mouse anesthetized with 1% isoflurane, shaving it, and taping it in a supine position to a heated EKG board where the image acquisition will take place. The image acquisition step consists of learning to identify the cardiac structures and obtaining all the required images with its correspondent probe and axis in order to be able to calculate volumes and diameters. The image analysis step consists of measuring the previously acquired images with the aid of computer software.Advantages of the proposed technique include a fast (15 min) procedure that would allow the researcher to evaluate interventions in a non-invasive, non-terminal approach and therefore follow the same mouse over time; each mouse can be used as its own control. This fact plus having the same operator perform all the acquisition and analysis for the entire experiment minimizes the limitation of operator-dependency. The present methodology is useful for mouse researchers in cardiovascular and pulmonary medicine.