Distance measurements in model bis-Gd(III) complexes with flexible "bridge". Emulation of biological molecules having flexible structure with Gd(III) labels attached.
Distance measurements in model bis-Gd(III) complexes with flexible "bridge". Emulation of biological molecules having flexible structure with Gd(III) labels attached.
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DOI：
10.1016/j.jmr.2010.03.019
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发表时间：
2010-07
期刊：
影响因子：
--
通讯作者：
Raitsimring A
中科院分区：
文献类型：
--
作者：
Potapov A;Song Y;Meade TJ;Goldfarb D;Astashkin AV;Raitsimring A
在这项工作中，我们继续探索Gd（III）作为一个可能的自旋标记高场双电子电子共振（DEER）为基础的距离测量生物分子与灵活的几何形状。为此，使用具有柔性“桥”的双Gd（III）络合物作为模型。模型中的距离预计分布在5-26海里的范围内，使我们能够探测可达距离的最短极限，发现可达距离小至13海里。还对这些标签的距离上限进行了评估，发现约为60 mm。由于短距离限制变化，不同的脉冲持续时间设置可能会导致从DEER动力学推导出的分布函数出现明显差异。的优点，如在低温和高重复率同时进行测量的能力，使用非常短的泵浦和观察脉冲没有相互干扰，缺乏取向的选择性，以及缺点，如有限的mW工作频率范围和固有的较小振幅的振荡与偶极相互作用相比，氮氧自旋标签进行了讨论。最有可能使用氮氧化合物和Gd基标记进行距离测量将是互补的，这取决于问题的细节和仪器的可用性。
In this work, we continue to explore Gd(III) as a possible spin label for high field Double Electron Electron Resonance (DEER) based distance measurements in biological molecules with flexible geometry. For this purpose, a bis-Gd(III) complex with a flexible “bridge” was used as a model. The distances in the model were expected to be distributed in the range of 5-26 Å, allowing us to probe the shortest limits of accessible distances which were found to be as small as 13 Å. The upper distance limit for these labels was also evaluated and was found to be about 60 Å. Various pulse duration setups can result in apparent differences in the distribution function derived from DEER kinetics due to short distance limit variations. The advantages, such as the ability to perform measurements at cryogenic temperatures and high repetition rates simultaneously, the use of very short pumping and observation pulses without mutual interference, the lack of orientational selectivity, as well as the shortcomings, such as the limited mw operational frequency range and intrinsically smaller amplitude of oscillation related to dipolar interaction as compared with nitroxide spin labels are discussed. Most probably the use of nitroxide and Gd based labels for distance measurements will be complementary depending on the particulars of the problem and the availability of instrumentation.
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影响因子：
14.9
作者：
Cai Q;Kusnetzow AK;Hubbell WL;Haworth IS;Gacho GP;Van Eps N;Hideg K;Chambers EJ;Qin PZ
通讯作者：
Qin PZ
影响因子：
17.1
作者：
Bird, Gregory H.;Pornsuwan, Soraya;Schafmeister, Christian E.
通讯作者：
Schafmeister, Christian E.
影响因子：
--
作者：
BLEANEY, B;RUBINS, RS
通讯作者：
RUBINS, RS
影响因子：
2.2
作者：
Banham, J. E.;Baker, C. M.;Timmel, C. R.
通讯作者：
Timmel, C. R.
影响因子：
15
作者：
Borbat, PP;Mchaourab, HS;Freed, JH
通讯作者：
Freed, JH