Probing molecular interfaces using 2D magic-angle-spinning NMR on protein mixtures with different uniform labeling
Probing molecular interfaces using 2D magic-angle-spinning NMR on protein mixtures with different uniform labeling
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DOI：
10.1021/ja0479181
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发表时间：
2004-11-17
影响因子：
15
通讯作者：
Baldus, M
中科院分区：
文献类型：
--
作者：
Etzkorn, M;Böckmann, A;Baldus, M
介绍了在魔角旋转下直接研究分子界面的一般核磁共振策略。该方法是基于对含有自旋种X和Y （X:Y）的均匀但非均匀标记的分子混合物的光谱分析。对于（N-15:C-13）标记的样品，演示了NC， NHC和NHHC转移的使用。应用于（C-13:N-15）标记的二聚体形式的85个氨基酸蛋白Crh， NHHC方法揭示了微晶状态下各种单体-单体相互作用。
A general NMR strategy to directly study molecular interfaces under magic angle spinning is introduced. The approach is based on the spectroscopic analysis of uniformly, but heterogeneously, labeled molecular mixtures containing the spin species X and Y (X:Y). For the case of an (N-15:C-13) labeled sample, the use of NC, NHC, and NHHC transfers is demonstrated. Applied to the (C-13:N-15) labeled dimeric form of the 85 amino acid protein Crh, the NHHC approach reveals a variety of monomer-monomer interactions in the microcrystalline state.