Use of a repetitive mouse B2 element to identify transplanted mouse cells in mouse-chick chimeras.
Use of a repetitive mouse B2 element to identify transplanted mouse cells in mouse-chick chimeras.
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使用重复的小鼠 B2 元件来鉴定小鼠-雏鸡嵌合体中移植的小鼠细胞。
DOI：
10.1006/excr.1999.4401
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
Kirby,ML
中科院分区：
文献类型：
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作者：
Bollag,RJ;Crawford,KB;Stadt,H;Kumiski,D;Zdanowicz,M;Baptista,C;Herlea,V;Kirby,ML
在胚胎发育过程中监测细胞的迁移需要一个系统，在这个系统中，细胞可以在运动过程中被识别。一个很有前途的系统是通过将小鼠细胞移植到鸡卵胚中来产生嵌合体，以利用小鼠丰富的遗传变异。该技术的成功依赖于在鸡环境中以高特异性检测单个小鼠细胞的能力。小鼠B2家族的短散布元件存在于小鼠基因组中，拷贝数超过105，而基于杂交技术，该序列在鸡基因组中不存在。这种五个数量级的差异在小鼠细胞中产生了容易识别的信号，即使在主要是鸡的环境中也是如此。因此，B2重复探针对于鉴定小鼠-鸡嵌合体中的小鼠细胞的目的是高度有效的。
Monitoring the migrations of cells during embryonic development requires a system in which cells can be identifiedin situduring locomotion. One promising system involves the generation of chimeras by transplanting mouse cells into chick embryosin ovoto exploit the wealth of mouse genetic variants. The success of this technique relies on the ability to detect individual mouse cells in a chick environment with high specificity. The murine B2 family of short interspersed elements is present in the mouse genome at copy numbers in excess of 105, whereas this sequence is absent in the chick genome based on hybridization techniques. This differential of five orders of magnitude produces signals in mouse cells that are easily identified, even in an environment that is predominantly chick. Thus, the B2 repeat probe is highly effective for the purpose of identifying mouse cells in mouse–chick chimeras.