Impact of recent innovations in the use of mass cytometry in support of drug development.
Impact of recent innovations in the use of mass cytometry in support of drug development.
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DOI：
10.1016/j.drudis.2015.06.001
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发表时间：
2015-10
影响因子：
7.4
通讯作者：
Wisnewski AV
中科院分区：
文献类型：
--
作者：
Nassar AF;Ogura H;Wisnewski AV
飞行时间细胞计数 (CyTOF) 是一种用于单细胞实时分析的新技术。 CyTOF 是免疫学、血液学和肿瘤学等领域的重大进步。它以最小的信号重叠解析每个细胞的多个金属偶联探针，从而最大限度地从每个单独的样品中获得信息。 CyTOF 能够对正常和患病状态的细胞进行表型和功能分析。单细胞技术使研究人员能够在单细胞水平上测量药物的作用并更好地了解其作用机制。在这里，我们讨论用于分析单个生物细胞的新型仪器、支持药物开发的最新创新的影响以及 CyTOF 在药物分析中的重要作用。
Cytometry by time-of-flight (CyTOF) is a novel technology for the real-time analysis of single cells. CyTOF is a significant advance in fields including immunology, hematology, and oncology. It resolves multiple metal-conjugated probes per cell with minimal signal overlap, which maximizes the information obtained from each individual sample. CyTOF provides the ability to phenotypically and functionally profile cells from normal and diseased states. Single cell technologies enable researchers to measure the effects of a drug at the single cell level and better understand its mechanism of action. Here, we discuss novel instruments for the analysis of individual biological cells, the impact of recent innovations in support of drug development, and the important roles of CyTOF in drug profiling.