Overexpressing <i>Pleurotus ostreatus rho1b</i> results in transcriptional upregulation of the putative cellulolytic enzyme‐encoding genes observed in <i>ccl1</i> disruptants
Overexpressing <i>Pleurotus ostreatus rho1b</i> results in transcriptional upregulation of the putative cellulolytic enzyme‐encoding genes observed in <i>ccl1</i> disruptants
复制标题
过度表达平菇 rho1b 会导致在 ccl1 破坏体中观察到的假定纤维素分解酶编码基因的转录上调
DOI：
10.1111/1462-2920.15786
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发表时间：
2021
影响因子：
5.1
通讯作者：
Honda Yoichi
中科院分区：
文献类型：
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作者：
Okuda Nozomi;Nakazawa Takehito;Horii Masato;Wu Hongli;Kawauchi Moriyuki;Sakamoto Masahiro;Honda Yoichi
白腐菌中木质纤维素酶编码基因的转录表达模式因培养条件的不同而不同。最近的研究表明，在平菇木质素降解缺陷突变株中，13个可能的纤维素酶编码基因在山毛榉木屑上显著上调。然而，这种转录转变的触发机制仍不清楚。在这项研究中，我们确定了一种机制。我们先前的研究表明，组蛋白H3N-二甲基化组蛋白在赖氨酸4水平上可能影响这种转变；因此，我们分析了干扰物OFP中的表达模式。Ostreatus ccl1，它编码COMPASS复合体的一个可能的成分，介导甲基化。结果显示，13个纤维素酶编码基因中有5个上调。我们还发现，编码GTPase调控信号转导通路的Rho1b在Clec-1干扰剂和木质素分解缺陷菌株中上调。在20天的培养过程中，在过表达Rho1b的菌株中观察到至少5个纤维素酶编码基因中的3个上调，但没有观察到Incl1/Rho1b双基因干扰物。这些结果表明，Rho1b可能参与了在破碎剂中观察到的纤维分解酶编码基因的上调。此外，我们认为Mpk1b是蘑菇特有的有丝分裂原激活的蛋白激酶，其功能位于Rho1b的下游。
The transcriptional expression pattern of lignocellulolytic enzyme‐encoding genes in white‐rot fungi differs depending on the culture conditions. Recently, it was shown that 13 putative cellulolytic enzyme‐encoding genes were significantly upregulated in mostPleurotus ostreatusligninolysis‐deficient mutant strains on beech wood sawdust medium. However, the mechanisms by which this transcriptional shift is triggered remain unknown. In this study, we identified one mechanism. Our previous study implied that histone H3N‐dimethylation at lysine 4 level possibly affects the shift; therefore, we analysed the expression pattern in the disruptants ofP. ostreatus ccl1, which encodes a putative component of the COMPASS complex mediating the methylation. The results showed upregulation of 5 of the 13 cellulolytic enzyme‐encoding genes. We also found thatrho1b, encoding a putative GTPase regulating signal transduction pathways, was upregulated in theccl1disruptants and ligninolysis‐deficient strains. Upregulation of at least three of the five cellulolytic enzyme‐encoding genes was observed inrho1b‐overexpressing strains but not inccl1/rho1bdouble‐gene disruptants, during the 20‐day culture period. These results suggest that Rho1b may be involved in the upregulation of cellulolytic enzyme‐encoding genes observed in theccl1disruptants. Furthermore, we suggest that Mpk1b, a putativeAgaricomycetes‐specific mitogen‐activated protein kinase, functions downstream of Rho1b.