Methionine-enkephalin and vasopressin in SHR: effects of dehydration.
Methionine-enkephalin and vasopressin in SHR: effects of dehydration.
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SHR 中的甲硫氨酸脑啡肽和加压素：脱水的影响。
DOI：
10.1152/ajpregu.1986.250.6.r1007
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Brooks,DP
中科院分区：
文献类型：
--
作者：
Ota,K;Share,L;Crofton,JT;Brooks,DP
脑啡肽存在于垂体后叶，可改变抗利尿激素的分泌，在自发性高血压大鼠（SHR）中比在Wistar-Kyoto （WKY）大鼠中具有更大的降压作用。血浆蛋氨酸-脑啡肽浓度（PMet-Enk）的测定在人类中提供了模棱两可的结果，在大鼠中没有报道。我们开发了一种高度特异性和敏感性的Met-Enk放射免疫测定方法，并确定Met-Enk在大鼠体内循环，但PMet-Enk在SHR和WKY大鼠之间没有差异（分别为7.6 +/- 0.8和9.2 +/- 0.8 pg/ml）。缺水48 h可使SHR和WKY大鼠血浆抗利尿激素浓度（PADH）和24 h尿抗利尿激素排泄量（UADHV）增加，但PMet-Enk没有变化。在脱水和脱水状态下，SHR和WKY大鼠的PADH和UADHV均无差异。这些结果表明，循环Met-Enk不太可能对SHR患者高血压的维持起重要作用。也没有证据表明SHR比WKY大鼠分泌更多的抗利尿激素，这与之前的报道相反。
Enkephalins are found in the posterior pituitary, can alter vasopressin secretion, and have greater pressor effects in spontaneously hypertensive rats (SHR) than in Wistar-Kyoto (WKY) rats. Measurement of the plasma methionine-enkephalin concentration (PMet-Enk) has provided equivocal results in humans and has not been reported in rats. We have developed a highly specific and sensitive Met-Enk radioimmunoassay and determined that Met-Enk circulates in rats but that PMet-Enk is no different between SHR and WKY rats (7.6 +/- 0.8 and 9.2 +/- 0.8 pg/ml, respectively). Water deprivation for 48 h increased the plasma vasopressin concentration (PADH) and 24-h urinary vasopressin excretion (UADHV) in SHR and WKY rats, but PMet-Enk was not altered. There were no differences in PADH and UADHV between SHR and WKY rats in either the euhydrated or dehydrated state. These results suggest that it is unlikely that circulating Met-Enk contributes importantly to the maintenance of hypertension in SHR. There was also no evidence for a greater secretion of vasopressin in SHR than in WKY rats, in contrast to previous reports.