Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis
Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis
复制标题
贻贝锰超氧化物歧化酶和铜/锌超氧化物歧化酶的分子特征
DOI：
10.1016/j.fsi.2013.01.011
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发表时间：
2013-05-01
影响因子：
4.7
通讯作者：
Zhao, Jianmin
中科院分区：
文献类型：
--
作者：
Wang, Qing;Yuan, Zeyi;Zhao, Jianmin
从紫贻贝中克隆了编码锰超氧化物歧化酶(MG-MnSOD)和铜/锌超氧化物歧化酶(MG-CuZnSOD)的全长cDNA序列。镁锰超氧化物歧化酶和镁铜锌超氧化物歧化酶分别编码228个和211个氨基酸的多肽。序列分析表明，镁锰超氧化物歧化酶为线粒体锰超氧化物歧化酶，镁铜锌超氧化物歧化酶为胞内铜锌超氧化物歧化酶。多重比对分析表明，镁锰超氧化物歧化酶和镁铜锌超氧化物歧化酶序列具有分别在锰超氧化物歧化酶和铜锌超氧化物歧化酶中保守的共同特征。系统发育分析表明，MG-MnSOD与其他软体动物的MnSOD聚在一起，而MG-CuZnSOD与其他软体动物胞内的CuZnSOD聚在一起，系统发育距离较远。通过实时定量RT-PCR(QPCR)分析，在所有肝胰腺表达水平最高的组织中都检测到了mg-MnSOD和mg-CuZnSOD的转录本。细菌攻击后，血细胞中镁锰超氧化物歧化酶和镁铜锌超氧化物歧化酶的表达水平先升高后下降到原来的水平。在肝胰腺组织中，镁铜锌超氧化物歧化酶基因在72 h和96 h显著上调，而镁锰超氧化物歧化酶转录本水平无明显变化。因此，镁锰超氧化物歧化酶和镁铜锌超氧化物歧化酶的表达是可诱导的，它们可能参与了对细菌攻击的免疫应答。这些结果表明，这些超氧化物歧化酶可能在高氏沼虾的免疫防御系统中发挥重要作用，并可能参与该贻贝对氧化应激的保护作用。(C)2013爱思唯尔有限公司。保留所有权利。
The full-length cDNA sequences coding respectively for a manganese superoxide dismutase (Mg-MnSOD) and copper/zinc superoxide dismutase (Mg-CuZnSOD) were cloned from Mytilus galloprovincialis. Mg-MnSOD and Mg-CuZnSOD cDNAs encoded a polypeptide of 228 and 211 amino acids, respectively. Sequence analysis indicated Mg-MnSOD was a mitochondrial MnSOD and Mg-CuZnSOD was an intracellular CuZnSOD. Multiple alignment analysis showed that both Mg-MnSOD and Mg-CuZnSOD sequences had the common features conserved in MnSODs and CuZnSODs, respectively. Phylogenetic analysis revealed that Mg-MnSOD clustered together with MnSODs from other mollusks, whereas Mg-CuZnSOD clustered with other mollusk intracellular CuZnSODs with a wider phylogenetic distance. By quantitative real-time RT-PCR (qPCR) analysis, both Mg-MnSOD and Mg-CuZnSOD transcripts were detected in all tissues examined with the highest expression level in hepatopancreas. Following bacterial challenge, the expression level of Mg-MnSOD and Mg-CuZnSOD increased first and subsequently decreased to the original level in hemocytes. In hepatopancreas, Mg-CuZnSOD mRNA was up-regulated significantly at 72 h and 96 h post challenge, while the level of Mg-MnSOD transcript had no significant change. Therefore, Mg-MnSOD and Mg-CuZnSOD expressions were inducible and they were probably involved in the immune response against bacterial challenge. These results suggest that these SODs may play important roles in the immune defense system of M. galloprovincialis and perhaps contribute to the protective effects against oxidative stress in this mussel. (C) 2013 Elsevier Ltd. All rights reserved.