Host response to malaria during pregnancy:: Placental monocyte recruitment is associated with elevated β chemokine expression
Host response to malaria during pregnancy:: Placental monocyte recruitment is associated with elevated β chemokine expression
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DOI：
10.4049/jimmunol.170.5.2759
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发表时间：
2003-03-01
影响因子：
4.4
通讯作者：
Rogerson, SJ
中科院分区：
文献类型：
--
作者：
Abrams, ET;Brown, H;Rogerson, SJ
怀孕期间的疟疾与不良的出生结果，特别是低出生体重有关。最近，单核细胞浸润到胎盘绒毛间隙已被确定为低出生体重的关键危险因素。然而，疟疾诱导的趋化因子参与招募和激活胎盘单核细胞尚未确定。在这项研究中，我们确定了哪些趋化因子在胎盘疟疾期间升高，以及趋化因子表达与胎盘单核细胞浸润之间的关系。胎盘疟疾感染与三种β趋化因子，巨噬细胞炎性蛋白1（MIP-1）α（CCL 3），单核细胞趋化蛋白1（MCP-1; CCL 2）和I-309（CCL 1），以及一种α趋化因子IL-8（CXCL 8）的mRNA表达升高相关;所有这些都与胎盘绒毛间隙中的单核细胞密度相关。胎盘型疟疾患者的胎盘血浆MIP-1 α和IL-8浓度升高，并与胎盘单核细胞浸润相关。通过免疫组织化学，我们定位了疟疾感染胎盘中胎盘趋化因子的产生：一些但不是所有的装载疟原虫色素的母体巨噬细胞产生MIP-1 β和MCP-1，胎儿基质细胞产生MCP-1。总之，在疟疾中，局部胎盘产生的趋化因子增加，并且可能是胎盘中单核细胞积聚的重要触发因素。
Malaria during pregnancy is associated with poor birth outcomes, particularly low birth weight. Recently, monocyte infiltration into the placental intervillous space has been identified as a key risk factor for low birth weight. However, the malaria-induced chemokines involved in recruiting and activating placental monocytes have not been identified. In this study, we determined which chemokines are elevated during placental malaria and the association between chemokine expression and placental monocyte infiltration. Placental malaria infection was associated with elevations in mRNA expression of three beta chemokines, macrophage-inflammatory protein 1 (MIP-1) alpha (CCL3), monocyte chemoattractant protein 1 (MCP-1; CCL2), and I-309 (CCL1), and one alpha chemokine, IL-8 (CXCL8); all correlated with monocyte density in the placental intervillous space. Placental plasma concentrations of MIP-1alpha and IL-8 were increased in women with placental malaria and were associated with placental monocyte infiltration. By immunohistochemistry, we localized placental chemokine production in malaria-infected placentas: some but not all hemozoin-laden maternal macrophages produced MIP-1beta and MCP-1, and fetal stromal cells produced MCP-1. In sum, local placental production of chemokines is increased in malaria, and may be an important trigger for monocyte accumulation in the placenta.