Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles.
Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles.
复制标题
胰腺炎后恢复过程中胰腺基因表达揭示了独特的转录组谱。
DOI：
10.1038/s41598-018-19392-0
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发表时间：
2018-01-23
影响因子：
4.6
通讯作者：
Husain SZ
中科院分区：
文献类型：
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作者：
Boggs K;Wang T;Orabi AI;Mukherjee A;Eisses JF;Sun T;Wen L;Javed TA;Esni F;Chen W;Husain SZ
众所周知，在单次损伤后，如孤立性胰腺炎发作后，胰腺恢复迅速。然而，目前还不清楚在这种情况下胰腺的分子景观会发生什么变化。在蛙皮素过度刺激胰腺炎模型中，基于组织学外观，小鼠胰腺具有在一周内恢复的能力。在这项研究中，我们试图通过RNA测序（RNA-seq）来表征损伤后两周恢复胰腺的转录谱。我们发现，损伤后一周与基线相比有319个差异表达基因（DEG），两周后有53个DEG。其中40例（12.5%）DEG在第1 ~ 2周持续存在，13例在第2周新出现。在最高上调的DEG中有几种胰蛋白酶原基因（胰蛋白酶原4、5、12、15和16）。据我们所知，这是通过深度测序对胰腺恢复期间转录组的首次表征，并且它在分子基础上揭示了即使在明显的组织学分辨率之后，胰腺也在持续恢复。这些发现也提高了胰腺恢复后出现新转录组的可能性。
It is well known that pancreatic recovery after a single episode of injury such as an isolated bout of pancreatitis occurs rapidly. It is unclear, however, what changes are inflicted in such conditions to the molecular landscape of the pancreas. In the caerulein hyperstimulation model of pancreatitis, the murine pancreas has the ability to recover within one week based on histological appearance. In this study, we sought to characterize by RNA-sequencing (RNA-seq) the transcriptional profile of the recovering pancreas up to two weeks post-injury. We found that one week after injury there were 319 differentially expressed genes (DEGs) compared with baseline and that after two weeks there were 53 DEGs. Forty (12.5%) of the DEGs persisted from week one to week two, and another 13 DEGs newly emerged in the second week. Amongst the top up-regulated DEGs were several trypsinogen genes (trypsinogen 4, 5, 12, 15, and 16). To our knowledge, this is the first characterization of the transcriptome during pancreatic recovery by deep sequencing, and it reveals on a molecular basis that there is an ongoing recovery of the pancreas even after apparent histological resolution. The findings also raise the possibility of an emerging novel transcriptome upon pancreatic recovery.