Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season
Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season
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DOI：
10.3168/jds.s0022-0302(02)74296-3
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发表时间：
2002-09-01
影响因子：
3.5
通讯作者：
Nardone, A
中科院分区：
文献类型：
--
作者：
Bernabucci, U;Ronchi, B;Nardone, A
本研究旨在评估炎热季节是否会影响过渡期奶牛的氧化状态。使用了20头荷斯坦奶牛。其中11头奶牛在春天产仔；剩下的九头奶牛在夏天生产。从产犊前21天至产犊后35天评估氧化状态，测定血浆中的氧化标志物[谷胱甘肽过氧化物酶活性、硫醇群、活性氧代谢物和硫代巴比妥酸活性物质（TBARS）]和红细胞中的氧化标志物[谷胱甘肽过氧化物酶活性（GSH-Px-E）、细胞内硫醇（SH）、超氧化物歧化酶活性（SOD）和TBARS]。季节并没有改变血浆氧化标志物。相反，由于夏季温湿指数（73.2 2.5平均日THI），奶牛暴露在中度热应激（39.5 +/- 0.2℃直肠温度）下，其红细胞SOD、GSH-Px-E、SH和TBARS均高于春季奶牛，表明奶牛处于氧化应激状态。一些变化（例如SOD的增加）代表了奶牛对氧化应激增加的反应所发生的代偿性变化。在比较的条件下，利用血浆标志物检测热应激对过渡期奶牛氧化状态的影响并不能提供足够的信息来得出明确的结论。红细胞是研究高温环境下过渡期奶牛氧化状态的合适且敏感的模型。
The study was carried out to assess whether hot seasons affect the oxidative status of transition dairy cows. Twenty Holstein cows were utilized. Eleven of those cows gave birth in spring; the remaining nine cows gave birth in summer. Oxidative status was evaluated from 21 d before to 35 d after calving, determining oxidative markers in plasma [glutathione peroxidase activity, thiol groups, reactive oxygen metabolites, and thiobarbituric acid reactive substances (TBARS)] and in erythrocytes [glutathione peroxidase activity (GSH-Px-E), intracellular thiols (SH), superoxide dismutase activity (SOD) and TBARS]. The season did not modify plasma oxidative markers. In contrast, cows exposed to moderate heat stress (39.5 +/- 0.2degreesC rectal temperatures) due to summer temperature-humidity index (73.2 2.5 mean daily THI), showed higher erythrocyte SOD, GSH-Px-E, SH, and TBARS compared with spring cows, indicating a condition of oxidative stress in summer transition dairy cows. Some changes (the increase of SOD, for example), represent compensatory changes the cow is undergoing in response to increased oxidative stress. Under the conditions compared, the effect of heat stress on oxidative status in transition dairy cows by use of plasma markers does not give enough information to reach definitive conclusions. Erythrocytes are an appropriate and sensitive model to study the oxidative status of transition dairy cows exposed to hot environments.