Lamprey Variable Lymphocyte Receptors Mediate Complement-Dependent Cytotoxicity
Lamprey Variable Lymphocyte Receptors Mediate Complement-Dependent Cytotoxicity
复制标题
七鳃鳗可变淋巴细胞受体介导补体依赖性细胞毒性
DOI：
10.4049/jimmunol.1200876
复制
发表时间：
2013-02-01
影响因子：
4.4
通讯作者：
Li, Qingwei
中科院分区：
文献类型：
--
作者：
Wu, Fenfang;Chen, Liyong;Li, Qingwei
另一种适应性免疫系统存在于最基本的脊椎动物七鳃鳗和八目鳗中，它们是唯一幸存的无颌脊椎动物。这些类似鳗鱼的鱼使用富含亮氨酸的重复序列受体来识别Ag，而不是使用有颌脊椎动物中的Ig受体。我们报告，在日本七鳃鳗（七鳃鳗），可变淋巴细胞受体（VLR）B与C1 q和C3蛋白相互作用，介导补体依赖性细胞毒性的细菌和肿瘤细胞。基于免疫的裂解涉及VLRB和C1 q样蛋白复合物在靶细胞表面上的沉积、C3的活化和细胞壁完整性的最终破坏。在七鳃鳗VLRB和补体成分之间的功能相互作用的演示提供了证据，合作的先天性和适应性免疫反应的出现在脊椎动物进化的一个关键点，之前或平行于基于Ig的抗体和经典的补体激活途径的进化。免疫学杂志，2013，190：922-930。
An alternative adaptive-immune system is present in the most basal vertebrates-lampreys and hagfish-the only surviving jawless vertebrates. These eel-like fish use leucine-rich repeat-based receptors for Ag recognition instead of the Ig-based receptors used in jawed vertebrates. We report that in Japanese lamprey (Lampetra japonica), variable lymphocyte receptor (VLR)B interacts with C1q and C3 proteins to mediate complement-dependent cytotoxicity for bacteria and tumor cells. The immune-based lysis involves deposition of VLRB and C1q-like protein complex on the surface of target cells, activation of C3, and ultimate disruption of cell wall integrity. The demonstration of functional interaction between VLRB and complement components in lamprey provides evidence for the emergence of cooperative innate and adaptive-immune responses at a pivotal point in vertebrate evolution, before or in parallel with the evolution of Ig-based Abs and the classical complement-activation pathway. The Journal of Immunology, 2013, 190: 922-930.