OsHOP2 regulates the maturation of crossovers by promoting homologous pairing and synapsis in rice meiosis
OsHOP2 regulates the maturation of crossovers by promoting homologous pairing and synapsis in rice meiosis
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OsHOP2通过促进水稻减数分裂中的同源配对和突触来调节交叉的成熟
DOI：
10.1111/nph.15664
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发表时间：
2019
期刊：
影响因子：
9.4
通讯作者：
Cheng Zhukuan
中科院分区：
文献类型：
--
作者：
Shi Wenqing;Tang Ding;Shen Yi;Xue Zhihui;Zhang Fanfan;Zhang Chao;Ren Lijun;Liu Changzhen;Du Guijie;Li Yafei;Yan Changjie;Cheng Zhukuan
减数分裂重组与同源配对和联会密切相关。以往的研究表明同源配对蛋白2（HOP2）在同源配对和突触中起着重要作用。然而，HOP2调节交叉（CO）形成的机制尚未阐明。本研究利用遗传分析、免疫定位和超分辨率成像技术分析了OsHOP2在水稻减数分裂中的功能，发现OsHOP2在水稻减数分裂中通过促进同源配对和突触形成来介导CO的成熟，并干扰了Oshop2减数分裂中的全长配对、突触和CO的形成。此外，结构照明显微镜显示OsHOP2定位于染色质，并与轴向元件（AE）和中心元件（CE）表现出相当大的共定位。更重要的是，OsHOP2与联会复合体的横丝蛋白（ZEP 1）之间的相互作用进一步证明OsHOP2参与联会复合体（SC）结构的组装或稳定。OsHOP2基因在水稻减数分裂中的启动和CO指定过程正常，但成熟的CO数量明显减少，并且人类入侵增强子10（HEI10）10位点仅存在于突触区域，推测OsHOP2基因可能是一个全局调节子，在水稻减数分裂中协调同源配对、突触和减数分裂重组。
Meiotic recombination is closely linked with homologous pairing and synapsis. Previous studies have shown that HOMOLOGOUS PAIRING PROTEIN2 (HOP2), plays an essential role in homologous pairing and synapsis. However, the mechanism by which HOP2 regulates crossover (CO) formation has not been elucidated. Here, we show that OsHOP2 mediates the maturation of COs by promoting homologous pairing and synapsis in rice (Oryza sativa) meiosis.We used a combination of genetic analysis, immunolocalization and super‐resolution imaging to analyze the function of OsHOP2 in rice meiosis.We showed that full‐length pairing, synapsis and CO formation are disturbed inOshop2meiocytes. Moreover, structured illumination microscopy showed that OsHOP2 localized to chromatin and displayed considerable co‐localization with axial elements (AEs) and central elements (CEs). Importantly, the interaction between OsHOP2 and a transverse filament protein of synaptonemal complex (ZEP1), provided further evidence that OsHOP2 was involved in assembly or stabilization of the structure of the synaptonemal complex (SC). Although the initiation of recombination and CO designation occur normally inOshop2mutants, mature COs were severely reduced, and human enhancer of invasion 10 (HEI10)10 foci were only present on the synapsed region.Putting the data together, we speculate that OsHOP2 may serve as a global regulator to coordinate homologous pairing, synapsis and meiotic recombination in rice meiosis.