CD4+ T cells and the proinflammatory cytokines gamma interferon and interleukin-6 contribute to alveolar bone loss in mice
CD4+ T cells and the proinflammatory cytokines gamma interferon and interleukin-6 contribute to alveolar bone loss in mice
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DOI：
10.1128/iai.67.6.2804-2809.1999
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发表时间：
1999-06-01
影响因子：
3.1
通讯作者：
Roopenian, DC
中科院分区：
文献类型：
--
作者：
Baker, PJ;Dixon, M;Roopenian, DC
在这项研究中，我们使用小鼠模型来研究适应性免疫反应在口腔感染人革兰氏阴性厌氧菌牙龈卟啉单胞菌引起的牙槽骨丢失中的作用。严重的联合免疫缺陷小鼠，缺乏B和T淋巴细胞，表现出相当少的骨丢失比免疫功能正常的小鼠口腔感染后，这表明淋巴细胞有助于这一过程。在缺乏主要组织相容性复合体（MHC）II类反应性CD 4（+）T细胞的小鼠中，口服感染后的骨丢失减少，但在缺乏MWC I类反应性CD 8（+）T细胞或NK 1（+）T细胞的小鼠中，未观察到骨丢失的变化。缺乏细胞因子γ干扰素或白细胞介素-6的小鼠也表现出骨丢失减少。这些结果表明，适应性免疫反应，特别是CD 4（+）T细胞和它们分泌的促炎细胞因子，是牙龈卟啉单胞菌口腔感染引起的骨丢失的重要影响因素。这些研究也加强了小鼠口腔感染模型在解剖牙周病病理生物学方面的实用性。
In this study, we used a mouse model to examine the role of the adaptive immune response in alveolar bone loss induced by oral infection with the human gram-negative anaerobic bacterium Porphyromonas gingivalis. Severe combined immunodeficient mice, which lack B and T lymphocytes, exhibited considerably less bone loss than did immunocompetent mice after oral infection, suggesting that lymphocytes contribute to this process. Bone loss after oral infection was decreased in mice deficient in major histocompatibility complex (MHC) class II-responsive CD4(+) T cells, but no change in bone loss was observed in mice deficient in MWC class I-responsive CD8(+) T cells or NK1(+) T cells. Mice lacking the cytokine gamma interferon or interleukin-6 also demonstrated decreased bone loss. These results suggest that the adaptive immune response, and in particular CD4(+) T cells and the proinflammatory cytokines that they secrete, are important effecters of bone loss consequent to P. gingivalis oral infection. The studies also reinforce the utility of the mouse oral infection model in dissecting the pathobiology of periodontal disease.