THE 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE GENE FAMILY OF ARABIDOPSIS-THALIANA
THE 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE GENE FAMILY OF ARABIDOPSIS-THALIANA
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DOI：
10.1073/pnas.89.22.11046
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发表时间：
1992-11-15
影响因子：
11.1
通讯作者：
THEOLOGIS, A
中科院分区：
文献类型：
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作者：
LIANG, XW;ABEL, S;THEOLOGIS, A
利用简并寡核苷酸引物从拟南芥基因组DNA中扩增出异源cDNA和PCR片段，从拟南芥中分离出5个ACC合成酶多肽（ACC 1、ACC 2、ACC 3、ACC 4和ACC 5）的基因组序列。每个基因位于拟南芥基因组中不同的染色体上。这些基因在发育过程中和对环境刺激的反应中差异表达。蛋白质合成抑制解除所有基因的表达，但最显着的解除抑制ACC 2，这表明它们的表达可能是负控制。测定了ACC 2基因的序列，并确定了其转录起始位点。通过在大肠杆菌中的表达实验证实了由该基因编码的多肽的真实性。该蛋白的预测大小为55，623 Da，并且它包含来自各种植物物种的转氨酶和ACC酶之间保守的11个不变氨基酸残基。拟南芥和其他植物ACC合酶基因的结构和表达特征的比较分析表明，ACC合酶基因的序列分歧和可能的不同的调控网络的ACC合酶亚家族的表达出现在植物进化的早期和之前的分歧单子叶植物和双子叶植物。
Genomic sequences encoding five divergent 1-aminocyclopropane-1-carboxylic acid (ACC) synthase polypeptides (ACC1, ACC2, ACC3, ACC4, and ACC5) have been isolated from Arabidopsis thaliana by using heterologous cDNAs and PCR fragments amplified from genomic DNA with degenerate oligonucleotide primers. Each gene is located on a different chromosome in the Arabidopsis genome. The genes are differentially expressed during development and in response to environmental stimuli. Protein-synthesis inhibition derepresses the expression of all genes but most dramatically derepresses that of ACC2, suggesting that their expression may be under negative control. The sequence of ACC2 was determined, and its transcription initiation site was defined. Authenticity of the polypeptide encoded by the gene was confirmed by expression experiments in Escherichia coli. The predicted size of the protein is 55,623 Da, and it contains the 11 invariant amino acid residues conserved between aminotransferases and ACC synthases from various plant species. Comparative analysis of structural and expression characteristics of ACC synthase genes from Arabidopsis and other plant species suggests that the sequence divergence of the ACC synthase genes and possibly the distinct regulatory networks governing the expression of ACC synthase subfamilies arose early in plant evolution and before the divergence of monocots and dicots.