Distribution of the Coenzyme M Pathway of Epoxide Metabolism among Ethene- and Vinyl Chloride-Degrading Mycobacterium Strains
Distribution of the Coenzyme M Pathway of Epoxide Metabolism among Ethene- and Vinyl Chloride-Degrading Mycobacterium Strains
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乙烯和氯乙烯降解分枝杆菌菌株环氧化物代谢辅酶 M 途径的分布
DOI：
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发表时间：
2003
影响因子：
4.4
通讯作者：
J. Spain
中科院分区：
文献类型：
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作者：
N. Coleman;J. Spain
摘要最近发现一种环氧烷烃：辅酶M（CoM）转移酶（EaCoMT）在分枝杆菌菌株JS 60的需氧氯乙烯（VC）和乙烯同化途径中具有活性。在本研究中，EaCoMT活性和基因进行了研究，在10个不同的分枝杆菌分离VC或乙烯从不同的环境样品。在所有情况下，细胞提取物中的环氧乙烷代谢依赖于CoM，EaCoMT的平均比活性在380和2，910 nmol/min/mg蛋白质之间。用基于来自分枝杆菌菌株JS 60和丙烯氧化剂黄曲霉菌株Py 2和红球菌菌株B-276的EaCoMT基因的保守区的引物进行PCR，从所有VC-和乙烯-同化分离物中产生EaCoMT基因的片段（834 bp）。分枝杆菌EaCoMT基因形成独特的簇，并且与红球菌菌株B-276的EaCoMT的相关性比与黄曲霉菌株Py 2的相关性更密切。EaCoMT和16 S rRNA基因树的不一致性以及来自地理位置遥远的分离株具有几乎相同的EaCoMT基因的事实表明，分支杆菌菌株之间发生了EaCoMT的横向转移。脉冲场凝胶电泳显示大的线性质粒（110至330 kb）在所有的VC降解菌株。在Southern印迹实验中，菌株JS 60 EaCoMT基因与许多质粒杂交。CoM介导的环氧化物代谢途径似乎是普遍的烯烃同化分枝杆菌，可能是因为质粒介导的横向基因转移。
ABSTRACT An epoxyalkane:coenzyme M (CoM) transferase (EaCoMT) enzyme was recently found to be active in the aerobic vinyl chloride (VC) and ethene assimilation pathways of Mycobacterium strain JS60. In the present study, EaCoMT activity and genes were investigated in 10 different mycobacteria isolated on VC or ethene from diverse environmental samples. In all cases, epoxyethane metabolism in cell extracts was dependent on CoM, with average specific activities of EaCoMT between 380 and 2,910 nmol/min/mg of protein. PCR with primers based on conserved regions of EaCoMT genes from Mycobacterium strain JS60 and the propene oxidizers Xanthobacter strain Py2 and Rhodococcus strain B-276 yielded fragments (834 bp) of EaCoMT genes from all of the VC- and ethene-assimilating isolates. The Mycobacterium EaCoMT genes form a distinct cluster and are more closely related to the EaCoMT of Rhodococcus strain B-276 than that of Xanthobacter strain Py2. The incongruence of the EaCoMT and 16S rRNA gene trees and the fact that isolates from geographically distant locations possessed almost identical EaCoMT genes suggest that lateral transfer of EaCoMT among the Mycobacterium strains has occurred. Pulsed-field gel electrophoresis revealed large linear plasmids (110 to 330 kb) in all of the VC-degrading strains. In Southern blotting experiments, the strain JS60 EaCoMT gene hybridized to many of the plasmids. The CoM-mediated pathway of epoxide metabolism appears to be universal in alkene-assimilating mycobacteria, possibly because of plasmid-mediated lateral gene transfer.
环氧烷烃：CoM 转移酶（一种锌依赖性环氧酶）中底物和辅因子相互作用的动力学和微量热分析。
影响因子：
2.9
作者：
Krum,JonathanG;Ellsworth,Heather;Sargeant,RyanR;Rich,Gregory;Ensign,ScottA
通讯作者：
Ensign,ScottA