In vitro effects of recombinant zebrafish IFN on spring viremia of carp virus and infectious hematopoietic necrosis virus.
In vitro effects of recombinant zebrafish IFN on spring viremia of carp virus and infectious hematopoietic necrosis virus.
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DOI：
10.1089/jir.2006.26.256
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发表时间：
2006-04
期刊：
影响因子：
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通讯作者：
Li Wang;Lei Wang;He-Xiao Zhang;Jian-hua Zhang;Wei-hong Chen;Xiaofei Ruan;Chun-Yan Xia
中科院分区：
文献类型：
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作者：
Li Wang;Lei Wang;He-Xiao Zhang;Jian-hua Zhang;Wei-hong Chen;Xiaofei Ruan;Chun-Yan Xia
为了研究重组鱼干扰素（recombinant interferon，rIFN）对鱼类致病性弹状病毒的作用，克隆了斑马鱼（Danio rerio）干扰素（DreIFN）等位基因B基因，并在大肠杆菌中表达。此外，在鱼和鸡细胞中研究了重组DreIFN（rDreIFN）对鲤鱼春季病毒血症病毒（SVCV）、传染性造血细胞坏死病毒（IHNV）和水泡性口炎病毒（VSV）的作用。DreIFN等位基因B基因的成熟肽编码163个氨基酸。残基3和98是可能形成链内二硫桥的一对半胱氨酸。SDS-PAGE检测到rDreIFN蛋白在21.6kDa处为条带。纯化的rDreIFN具有3 × 10（4）U/mg-10（7）U/mg的抗SVCV和抗IHNV活性。结果表明，rDreIFN对鲤上皮瘤病毒（EPC）的抗SVCV和IHNV活性高于草鱼（C。Idellus）卵巢（CO）细胞系的抗VSV活性，而对鸡胚成纤维细胞（CEF）无抗VSV活性。
In order to evaluate the effects of fish recombinant interferon (rIFN) on fish pathogenic rhabdoviruses, the zebrafish (Danio rerio) IFN (DreIFN) allele B gene was cloned and expressed in Escherichia coli. In addition, the effects of recombinant DreIFN (rDreIFN) on spring viremia of carp virus (SVCV), infectious hematopoietic necrosis virus (IHNV), and vesicular stomatitis virus (VSV) were surveyed in fish and chicken cells. The mature peptide of DreIFN allele B gene encodes 163 amino acids. Residues 3 and 98 are a pair of cysteines that likely form an intrachain disulfide bridge. rDreIFN protein was detected as a band at 21.6 kDa by SDS-PAGE. The purified rDreIFN has anti-SVCV and anti-IHNV activity of 3 x 10(4) U/mg-10(7) U/mg. The results indicate that rDreIFN has higher activity against SVCV and IHNV on epithelioma papulosum cyprinid (EPC) than on grass carp (C. idellus) ovary (CO) cell lines and no activity against VSV on chick embryo fibroblasts (CEF).