Role for trehalase during germination of spores in the fission yeast Schizosaccharomyces pombe
Role for trehalase during germination of spores in the fission yeast Schizosaccharomyces pombe
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DOI：
10.1016/s0378-1097(00)00471-7
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发表时间：
2000-12-01
影响因子：
2.1
通讯作者：
Gacto, M
中科院分区：
文献类型：
--
作者：
Beltran, FF;Castillo, R;Gacto, M
裂糖菌孢子含有中性和酸性海藻酶。当来自编码中性海藻酶的ntp1（+）的菌株的孢子被诱导发芽时，与野生型孢子相比，该过程的开始明显延迟。进一步的增长也减少了。缺乏中性海藻糖酶的休眠孢子所含的海藻糖量是野生型孢子的两倍，并且在萌发过程中细胞内海藻糖池的动员速度较慢。在ntp1破坏的孢子中，根霉苷对孢子特异性酸性海藻化酶的抑制完全阻止了孢子的萌发，而对野生型孢子没有影响。这些结果表明，这两种海藻糖酶可能支持海藻糖在萌发过程中的利用，但需要中性海藻糖酶才能更快速有效地利用海藻糖。(C) 2000年欧洲微生物学会联合会。Elsevier Science B.V.版权所有。
Spores from Schizosaccharomyces pombe contain neutral and acid trehalases. When spores from strains disrupted for ntp1(+), which encodes neutral trehalase, were induced to germinate, the onset of the process was markedly delayed as compared to wild-type spores. Further outgrowth was also reduced. Dormant spores lacking neutral trehalase contained twice the amount of trehalose present in wild-type spores and mobilised the intracellular pool of trehalose at a slower rate during germination. Inhibition by phloridzin of the sporulation-specific acid trehalase in ntp1-disrupted spores arrested germination completely while prompting no effect on wild-type spores. These results suggest that the two trehalase enzymes may support the utilisation of trehalose during germination but neutral trehalase is required for a more rapid and efficient process. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.