Preparation and assay of monohydroxy-eicosatetraenoic acids.
Preparation and assay of monohydroxy-eicosatetraenoic acids.
复制标题
单羟基二十碳四烯酸的制备和测定。
DOI：
10.1016/0003-2697(80)90073-1
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发表时间：
1980
影响因子：
2.9
通讯作者：
Hubbard,WC
中科院分区：
文献类型：
--
作者：
Boeynaems,JM;Brash,AR;Oates,JA;Hubbard,WC
以花生四烯酸为原料，在Cu2+存在下与过氧化氢反应合成了5-，8-，9-，11-，12-和15-羟基二十碳四烯酸(HETE)。在硅胶(μPorasil)上，使用从正己烷到氯仿的线性溶剂梯度法分离它们：只有8-和9-异构体不被拆分。这种方法可以很容易地产生数毫克量的高纯度HETE，从而为研究这些化合物的生物活性提供了有用的工具。以八氢花生四烯酸为原料制备的HETEs的八氢类似物适合作为稳定同位素稀释分析的内标，采用气相色谱-质谱法相结合的方法，选择离子监测。HETES的检出限小于1 ng。
5-, 8-, 9-, 11-, 12-, and 15-hydroxy-eicosatetraenoic acids (HETEs) were prepared from arachidonic acid by reaction with H2O2in the presence of Cu2+ions. They were separated by high-performance liquid chromatography on silica gel (μPorasil), using a linear solvent gradient from hexane to chloroform: only the 8- and 9-isomers were not resolved. Multi-milligram quantities of highly purified HETEs could be easily generated by this method, which thus provides a useful tool to study the biological activity of these compounds. Octadeuterated analogs of HETEs prepared from octadeuterated arachidonic acid by this procedure were suitable for use as internal standards in stable isotope dilution assays, by combined gas chromatography and mass spectrometry, with selected ion monitoring. The detection limit of the HETEs was less than 1 ng.