Species-Specific Differences in the Microbiomes and Organic Exudates of Crustose Coralline Algae Influence Bacterioplankton Communities
Species-Specific Differences in the Microbiomes and Organic Exudates of Crustose Coralline Algae Influence Bacterioplankton Communities
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DOI：
10.3389/fmicb.2019.02397
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发表时间：
2019-11-08
影响因子：
5.2
通讯作者：
Nelson, Craig E.
中科院分区：
文献类型：
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作者：
Quinlan, Zachary A.;Ritson-Williams, Raphael;Nelson, Craig E.
壳状珊瑚藻是珊瑚礁生态系统的重要组成部分，但对其研究甚少。最近关于CCA的研究表明，只有少数几个物种通过产生化学定居线索或促进被假设影响珊瑚定居的特定微生物群落，在珊瑚幼虫的定居中发挥重要作用。因此，定义如何DOM分泌物CCA物种和浮游细菌群落之间的差异，这些分泌物促进了解CCA在无脊椎动物定居的作用是很重要的。通过对两种CCA进行为期一天的分泌实验，比较了组织微生物群落结构、DOM产生量以及DOM对浮游细菌群落的影响。我们收集了来自Hydrolithon reinboldii和Porolithon onkodes的渗出物，分别来自夏威夷Kane'ohe湾的过滤灭菌海水和未过滤海水。我们的研究结果表明，虽然这两个物种渗出等量的溶解有机碳，他们不同的荧光DOM的组成和培养不同的微生物群落。P. onkodes渗出物促进更多与珊瑚病相关的微生物OTU，而H. reinboldii促进已知产生抗微生物化合物的OTU。我们的研究结果突出了CCA的fDOM渗出物的组成和微生物群落结构的影响的物种特异性差异。
Crustose coralline algae (CCA) are critical members of the coral reef ecosystem, yet they remain poorly studied. Recent research on CCA has shown that only a few species play a significant role in the settlement of coral larvae through either the production of chemical settlement cues or the facilitation of specific microbial communities that are hypothesized to influence coral settlement. Thus, defining how DOM exudates differ between CCA species and the bacterioplankton communities these exudates facilitate is important for understanding the role of CCA in invertebrate settlement. We conducted single day exudation experiments on two species of CCA to compare tissue microbiome community structure, DOM production and the effect of DOM on the bacterioplankton community. We collected exudates from Hydrolithon reinboldii and Porolithon onkodes in both filter-sterilized seawater and unfiltered seawater from Kane'ohe Bay, Hawai'i. Our results demonstrate that while both species exude equivalent quantities of dissolved organic carbon they differ in the composition of fluorescent DOM and fostered distinct microbial communities. P. onkodes exudates facilitate more microbial OTUs associated with coral disease, whereas H. reinboldii facilitated OTUs known to produce antimicrobial compounds. Our results highlight species-specific differences in the composition of fDOM exudates of CCA and the effect of those on microbial community structure.