Genomic structure and refined chromosomal localization of the mouse Ptch2 gene.
Genomic structure and refined chromosomal localization of the mouse Ptch2 gene.
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小鼠 Ptch2 基因的基因组结构和精细染色体定位。
DOI：
10.1159/000064064
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发表时间：
2002
影响因子：
1.7
通讯作者：
Lanske,B
中科院分区：
文献类型：
--
作者：
Fröhlich,L;Liu,Z;Beier,DR;Lanske,B
脊椎动物Patched 2（Ptch 2）基因编码一种假定的膜包埋蛋白，该蛋白可能在发育和肿瘤发生过程中的Hedgehog信号传导中发挥作用。我们确定了小鼠Ptch 2基因的基因组结构，并表明Ptch 2由22个外显子组成，跨越约18 kb的基因组DNA。发现外显子-内含子边界在人和小鼠Ptch 2基因内是保守的。5′侧翼区的分析显示了一个CpG岛，推测的启动子区和转录起始位点，而在3′侧翼区鉴定了一个多聚腺苷酸化信号和mRNA去稳定化基序。利用单链构象多态性分析将小鼠Ptch 2定位于4号染色体微卫星标记D4 Mit 20和D4 Mit 334之间。
The vertebrate Patched 2 (Ptch2) gene encodes a putative membrane-embedded protein which may have roles in Hedgehog signaling during development and in tumorigenesis. We determined the genomic structure of the mouse Ptch2 gene and show that Ptch2 is composed of 22 exons spanning approximately 18 kb of genomic DNA. The exon-intron boundaries were found to be conserved within the human and mouse Ptch2 genes. Analysis of the 5′ flanking region revealed a CpG island, the putative promoter region and the transcriptional start site while a polyadenylation signal as well as a mRNA destabilizing motif were identified in the 3′ flanking region. Single-strand conformation polymorphism analysis was used to map mouse Ptch2 to chromosome 4 between the microsatellite markers D4Mit20 and D4Mit334.