Exposure to 50 Hz electromagnetic fields enhances hair follicle regrowth in C57BL/6 mice
Exposure to 50 Hz electromagnetic fields enhances hair follicle regrowth in C57BL/6 mice
复制标题
暴露于 50Hz 电磁场可增强 C57BL/6 小鼠的毛囊再生
DOI：
10.1177/1535370219834639
复制
发表时间：
2019-04-01
影响因子：
3.2
通讯作者：
Zhang, Mingsheng
中科院分区：
文献类型：
--
作者：
Li, Xinping;Wang, Xin;Zhang, Mingsheng
许多研究表明，电磁场活动影响参与毛囊形成的多种细胞的细胞活动。然而，电磁场对毛囊生长的生物效应尚未完全阐明。本研究旨在确定50赫兹电磁场是否能促进毛囊再生。在本实验中，使用C57BL/6小鼠建立脱毛诱导的毛囊周期模型，然后将这些小鼠随机分为对照组和电磁场组。在电磁场（50赫兹，5毫特斯拉）暴露16天后，采集小鼠皮肤样本以评估毛发再生情况，并通过免疫荧光染色评估表皮干细胞增殖情况。还检测了角质形成细胞生长因子的表达和定位。我们的结果显示，与对照组相比，电磁场组在脱毛后第3天毛球形成更快，在第9天大部分毛干更早地从表皮毛孔中长出，并且在第16天毛发长度明显更长。经过电磁场处理后，与对照组相比，在外根鞘和毛球中与K15（+）细胞共定位的Ki67（+）细胞更多。两组的内根鞘中都有角质形成细胞生长因子表达，且电磁场组角质形成细胞生长因子表达更多。我们的数据表明，在脱毛诱导的毛囊周期中观察到50赫兹电磁场有促进毛发生长的作用，这与50赫兹电磁场增强K15（+）干细胞增殖以及角质形成细胞生长因子表达增加有关。
影响声明
在本研究中，我们的实验证实50赫兹电磁场影响毛囊再生，并且50赫兹电磁场增强毛囊的毛球和毛囊外根鞘中K15（+）干细胞的增殖。这些结果表明50赫兹电磁场可能对脱发的功能性修复有益。
Many studies have suggested that electromagnetic field activity affects the cellular activity of many types of cells involved in forming hair follicles. However, the bio-effects of electromagnetic fields on hair follicle growth have not been fully elucidated. This present study was designed to determine whether 50 Hz electromagnetic fields increased hair follicle regrowth. In this experiment, C57BL/6 mice were used to present the model of depilation-induced hair follicle cycling, and then those mice were divided at random into the control group and the electromagnetic field group. After electromagnetic field (50 Hz, 5 mT) exposure for 16 days, the skin specimens of the mice were harvested to assess for hair regrowth, and epidermal stem cells proliferation was evaluated by immunofluorescence staining. The expression and location of keratinocyte growth factors were also tested. Our results showed that, compared to the control, the hair club formed faster on the 3rd day, and most of the hair shafts erupted earlier from the pore in the epidermis on the 9th day after depilation, and the hairs length was significantly longer on the 16th day within the electromagnetic field group. After electromagnetic field treatment, there were more Ki67(+) cells in the outer root sheath and hair bulb where it co-localized with K15(+) cells compared to the control. Keratinocyte growth factors were expressed in the inner root sheath in both groups, and the electromagnetic field group showed more expression of keratinocyte growth factors. Our data suggested that the hair-growth-promoting effect of the 50 Hz electromagnetic field was observed in depilation-induced hair follicles cycling, which was associated with 50 Hz electromagnetic field enhancing K15(+) stem cells proliferation and increased keratinocyte growth factor expression. Impact statement In this study, our experiments confirmed that 50 Hz EMF affected hair follicle regrowth, and 50 Hz EMF enhanced K15(+) stem cells proliferation in the hair bulb and follicular outer root sheath of hair follicles. Those results indicated that 50 Hz EMF may be beneficial for functional healing of hair loss.