A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation
A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation
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DOI：
10.1016/s1097-2765(00)80178-1
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发表时间：
1999-01-01
期刊：
影响因子：
16
通讯作者：
Roeder, RG
中科院分区：
文献类型：
--
作者：
Gu, W;Malik, S;Roeder, RG
一种新的人类复合体已经纯化，它既可以抑制PC4介导的激活剂依赖的转录，也可以在限制TFIIH的情况下，与PC4协同作用增强激活剂依赖的转录。该复合体包含酵母介体/全酶组分(包括SRB7、SRB10、SRB11、MED6和RGR1)的子集的同源物，其他酵母转录调节因子的同源物(SOH1和NUT2)，以及值得注意的是，人甲状腺激素受体相关辅活化子复合体的一些成分(TRAP220、TRAP170/hRGR1和TRAP100)。该复合体表现出直接的激活剂相互作用，但与酵母介体不同，它可以独立于RNA聚合酶II CTD发挥作用。这些发现证明了人类复合体的积极和消极的功能，强调了新的(CTD非依赖性)调节机制，并将该复合体与其他人类共激活物复合体联系起来。
A novel human complex that can either repress activator-dependent transcription mediated by PC4, or, at limiting TFIIH, act synergistically with PC4 to enhance activator-dependent transcription has been purified. This complex contains homologs of a subset of yeast mediator/holoenzyme components (including SRB7, SRB10, SRB11, MED6, and RGR1), homologs of other yeast transcriptional regulatory factors (SOH1 and NUT2), and, significantly, some components (TRAP220, TRAP170/hRGR1, and TRAP100) of a human thyroid hormone receptor-associated coactivator complex. The complex shows direct activator interactions but, unlike yeast mediator, can act independently of the RNA polymerase II CTD. These findings demonstrate both positive and negative functional capabilities for the human complex, emphasize novel (CTD-independent) regulatory mechanisms, and link the complex to other human coactivator complexes.