Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation
Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation
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DOI：
10.1016/j.yexcr.2004.07.005
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发表时间：
2004-10-15
影响因子：
3.7
通讯作者：
Nishimoto, T
中科院分区：
文献类型：
--
作者：
Sekiguchi, T;Iida, H;Nishimoto, T
我们研究了Y染色体AZFa区域DDX3Y的功能，该区域编码一种假定的DEAD-box RNA解旋酶蛋白，该区域的缺失会导致人类少精子症或无精子症。人类DDX3Y氨基酸序列与X染色体基因DDX3X相似（同源性91.7%）。在这里，我们报告了人类Y和x编码的DEAD box RNA解旋酶蛋白DDX3Y和DDX3X是可互换的，并且具有基本功能：这两种蛋白都挽救了温度敏感突变的仓鼠细胞系（tsET24），否则在不允许的温度下无法生长。小鼠同源物Ddx3y和D1Pas1-PL10也在不允许的温度下拯救突变细胞系。原位杂交显示，Ddx3x mRNA在小鼠睾丸的几乎所有细胞中都有表达，表明Ddx3x参与了精子发生。通过比较DDX3X和DDX3Y来确定DDX3Y对细胞生长和精子发生的意义。DDX3X和DDX3Y启动子dna在体内产生相似程度的转录，而对启动子dna的缺失研究表明，这些基因受到不同的调控。与DDX3X类似，DDX3Y以依赖于crm1的方式在细胞核和细胞质之间穿梭。(C) 2004爱思唯尔公司版权所有。
We investigated the function of DDX3Y, the Y chromosome AZFa region encoding a putative DEAD-box RNA helicase protein, the loss of which results in oligozoospermia or azoospermia in humans. The human DDX3Y amino acid sequence is similar to that of the X chromosome gene DDX3X (91.7% homology). Here we report that human Y- and X-encoded DEAD box RNA helicase proteins DDX3Y and DDX3X are interchangeable and have an essential function: both proteins rescued a temperature-sensitive mutant hamster cell line (tsET24) that was otherwise incapable of growth at a nonpermissive temperature. Mouse homologues Ddx3y and D1Pas1-PL10 also rescued the mutant cell line at a nonpermissive temperature. In situ hybridization revealed that Ddx3x mRNA was expressed in almost every cell in mouse testis, suggesting that Ddx3x is involved in spermatogenesis. A comparative study of DDX3X and DDX3Y was performed to determine the significance of DDX3Y for cell growth and spermatogenesis. Both DDX3X and DDX3Y promoter DNAs produced a similar degree of transcription in vivo, whereas deletion studies of the promoter DNAs indicated that these genes are differentially regulated. DDX3Y, similar to DDX3X, shuttles between the nucleus and cytoplasm in a crm1-dependent manner. (C) 2004 Elsevier Inc. All rights reserved.