Effects of cell swelling on intracellular calcium and membrane currents in bovine articular chondrocytes.
Effects of cell swelling on intracellular calcium and membrane currents in bovine articular chondrocytes.
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细胞肿胀对牛关节软骨细胞内钙和膜电流的影响。
DOI：
10.1002/jcb.10217
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Donahue,HenryJ
中科院分区：
文献类型：
--
作者：
Yellowley,ClareE;Hancox,JulesC;Donahue,HenryJ
在正常关节负荷过程中，当液体被迫离开基质时，软骨细胞经历动态细胞外渗透环境，增加局部蛋白聚糖浓度，从而增加离子强度和渗透压。为了在这样一个具有挑战性的环境中生存，软骨细胞必须具有调节细胞体积的机制。在这项研究中，我们研究了牛关节软骨细胞（BAC）在低渗挑战过程中调节细胞体积的能力。我们还研究了低渗应激对早期信号事件的影响，包括[Ca 2 +] i和膜电流。通过监测负载钙黄绿素的细胞的荧光来测量细胞体积的变化。[Ca2+] i使用fura-2定量，并使用膜片钳记录膜电流。当暴露于低渗盐水时，BAC表现出调节性容量减少（RVD），Gd 3+可抑制该作用。肿胀刺激BAC内[Ca ~（2+）] i的变化，这种变化依赖于肿胀的大小。Gd ~（3+）、zero [Ca ~（2+）]o和毒胡萝卜素（thapsigargin）均减弱[Ca ~（2+）] i反应，提示Ca ~（2+）通过牵张激活通道内流和Ca ~（2+）从细胞内库释放的作用。细胞肿胀时内向和外向膜电流均显著增加，并被Gd ~（3+）抑制。这些结果表明，BAC的RVD可能涉及[Ca ~（2+）] i和离子通道激活，这两者在其他细胞类型的RVD中起关键作用。这些信号传导途径也类似于在软骨细胞中受到其他生物物理信号激活的那些。这是可能的，然后，这些信号事件也可能参与机械负荷被软骨细胞转化为适当的细胞反应的机制。J.细胞。86：290-301，2002.© 2002 Wiley利斯公司
Chondrocytes experience a dynamic extracellular osmotic environment during normal joint loading when fluid is forced from the matrix, increasing the local proteoglycan concentration and therefore the ionic strength and osmolarity. To exist in such a challenging environment, chondrocytes must possess mechanisms by which cell volume can be regulated. In this study, we investigated the ability of bovine articular chondrocytes (BAC) to regulate cell volume during a hypo‐osmotic challenge. We also examined the effect of hypo‐osmotic stress on early signaling events including [Ca2+]iand membrane currents. Changes in cell volume were measured by monitoring the fluorescence of calcein‐loaded cells. [Ca2+]iwas quantified using fura‐2, and membrane currents were recorded using patch clamp. BAC exhibited regulated volume decrease (RVD) when exposed to hypo‐osmotic saline which was inhibited by Gd3+. Swelling stimulated [Ca2+]itransients in BAC which were dependent on swelling magnitude. Gd3+, zero [Ca2+]o, and thapsigargin all attenuated the [Ca2+]iresponse, suggesting roles for Ca2+influx through stretch activated channels, and Ca2+release from intracellular stores. Inward and outward membrane currents significantly increased during cell swelling and were inhibited by Gd3+. These results indicate that RVD in BAC may involve [Ca2+]iand ion channel activation, both of which play pivotal roles in RVD in other cell types. These signaling pathways are also similar to those activated in chondrocytes subjected to other biophysical signals. It is possible, then, that these signaling events may also be involved in a mechanism by which mechanical loads are transduced into appropriate cellular responses by chondrocytes. J. Cell. Biochem. 86: 290–301, 2002. © 2002 Wiley‐Liss, Inc.
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耐受和身体依赖性大鼠吗啡戒断后，用 [3H]SCH 23390 标记的脑和脊髓多巴胺 D1 受体的修饰。
DOI：
--
发表时间：
1990
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Bhargava,HN;Gulati,A
通讯作者：
Gulati,A
3H-纳洛酮在小鼠大脑中的结合：离子的影响和耐受性的发展。
DOI：
--
发表时间：
1974
期刊：
Life Science
影响因子：
--
作者：
Robert Hitzemann;Barbara A. Hitzemann;Horace H. Loh
通讯作者：
Horace H. Loh
慢性吗啡上调由[3H]cycloFOXY标记的μ阿片结合位点：一种适用于正电子发射断层扫描的新型阿片拮抗剂。
影响因子：
5
作者：
R. Rothman;S. Mclean;V. Bykov;R. Lessor;A. Jacobson;K. Rice;J. Holaday
通讯作者：
J. Holaday
大鼠体内颗粒植入诱导的吗啡耐受性的定量
影响因子：
3.3
作者：
H. Bhargava
通讯作者：
H. Bhargava
阿片受体结合——体内阿片给药增强。
影响因子：
5.8
作者：
C. Pert;S. Snyder
通讯作者：
S. Snyder