Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize
Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize
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DOI：
10.1007/s00438-007-0289-y
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发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Rafalski, Antoni
中科院分区：
文献类型：
--
作者：
Belo, Andre;Zheng, Peizhong;Rafalski, Antoni
我们利用全基因组扫描关联图谱鉴定了对玉米籽粒油酸含量有重要影响的位点。对553份玉米自交系8590个位点的单核苷酸多态性单倍型与油酸含量的关系进行了检测。在4号染色体IBM2邻居遗传图谱中，在380 ~ 384 cM之间定位了一个对油酸有主要影响的单位点，并在双亲本群体中得到了证实。脂肪酸去饱和酶fad2，从相关遗传标记中鉴定出与2kb相似，是最有可能导致表型差异的候选基因。对高油酸含量和低油酸含量的fad2等位基因进行了测序，并研究了发育胚胎中fad2 RNA水平的等位基因差异。我们认为fad2活性位点附近的非保守氨基酸多态性对油酸含量有影响。这是第一个使用高分辨率全基因组扫描关联图谱的报告，其中一个假定的基因负责植物的数量性状。
We used whole genome scan association mapping to identify loci with major effect on oleic acid content in maize kernels. Single nucleotide polymorphism haplotypes at 8,590 loci were tested for association with oleic acid content in 553 maize inbreds. A single locus with major effect on oleic acid was mapped between 380 and 384 cM in the IBM2 neighbors genetic map on chromosome 4 and confirmed in a biparental population. A fatty acid desaturase, fad2, identified similar to 2 kb from the associated genetic marker, is the most likely candidate gene responsible for the differences in the phenotype. The fad2 alleles with high- and low-oleic acid content were sequenced and allelic differences in fad2 RNA level in developing embryos was investigated. We propose that a non-conservative amino acid polymorphism near the active site of fad2 contributes to the effect on oleic acid content. This is the first report of the use of a high resolution whole genome scan association mapping where a putative gene responsible for a quantitative trait was identified in plants.