Acute effects of arterial baroreflex on sympathetic nerve activity and plasma norepinephrine concentration
Acute effects of arterial baroreflex on sympathetic nerve activity and plasma norepinephrine concentration
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DOI：
10.1016/j.autneu.2014.10.016
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发表时间：
2014-12-01
影响因子：
2.7
通讯作者：
Sugimachi, Masaru
中科院分区：
文献类型：
--
作者：
Kawada, Toru;Akiyama, Tsuyoshi;Sugimachi, Masaru
动脉压(AP)与外源性给药剂量去甲肾上腺素(NE)呈对数函数关系。相反，在急性压力感受性反射干预中，AP与传出交感神经活动(SNA)几乎呈线性相关。本研究旨在量化在急性压力反射干预过程中SNA与血浆NE浓度的关系。将5只Wistar京都大鼠的颈动脉窦区从体循环中分离出来，颈动脉窦压力每2分钟变化一次，范围分别为60、100、120、140和180 mm Hg。各压力水平取动脉血0.2ml，测定血浆去甲肾上腺素。压力扰动的最大AP为153.34±-6.28 mm Hg，最小AP为67.31±-4.92 mm Hg。对于个体动物数据，血浆NE与SNA呈线性相关(斜率：0.957+/-0.090 pg.ml(-1).%(-1)，截距：46.57+/-7.22pg/ml.r(2)：范围为0.923-0.992)，对于群体平均数据(NE=0.956 x SNA+47.97，r(2)=0.982)。静脉注射地昔帕明(1 mg/kg)阻断神经元对NE的摄取，使血浆NE-SNA关系的斜率(168.73±28.53pg.ml(-1).%)和截距(2.966±0.686)pg.ml(-1)增加。这些结果表明，在AP急性压力感受性反射控制的正常生理范围内，SNA与血浆NE浓度几乎呈线性关系。虽然血浆NE浓度可以反映SNA的变化，但它也可能高估了神经元NE摄取全身性受损时交感神经流出中枢神经系统的程度。(C)2014爱思唯尔B.V.保留所有权利。
Arterial pressure (AP) elevates as a logarithmic function of exogenously administered dose of norepinephrine (NE). In contrast, AP is nearly linearly correlated with efferent sympathetic nerve activity (SNA) during acute baroreflex intervention. The present study aimed at quantifying the relationship between SNA and plasma NE concentration during acute baroreflex intervention. Carotid sinus regions were isolated from systemic circulation in five Wistar Kyoto rats, and carotid sinus pressure was changed among 60, 100, 120, 140, and 180 mm Hg every 2 min. Arterial blood (0.2 ml) was obtained at each pressure level for plasma NE measurement. Maximum AP and minimum AP were 153.34 +/- 6.28 and 67.31 +/- 4.92 mm Hg, respectively, in response to pressure perturbation. Plasma NE correlated linearly with SNA for individual animal data (slope: 0.957 +/- 0.090 pg.ml(-1).%(-1), intercept: 46.57 +/- 7.22 pg/ml.r(2): ranged from 0.923 to 0.992) and also for group averaged data (NE = 0.956 x SNA + 47.97, r(2) = 0.982). Blockade of neuronal NE uptake by intravenous desipramine (1 mg/kg) administration increased the slope (2.966 +/- 0.686 pg.ml(-1).%(-1) P < 0.05) and the intercept (168.73 +/- 28.53 pg/ml, P < 0.01) of the plasma NE-SNA relationship. These results indicate that the relationship between SNA and plasma NE concentration was nearly linear within the normal physiological range of acute baroreflex control of AP. While plasma NE concentration can reflect changes in SNA, it may also overestimate the sympathetic outflow from the central nervous system when neuronal NE uptake is impaired systemically. (C) 2014 Elsevier B.V. All rights reserved.