Changes in plasma motilin concentration in response to manipulation of intragastric and intraduoduenal contents in man.
Changes in plasma motilin concentration in response to manipulation of intragastric and intraduoduenal contents in man.
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人胃内和十二指肠内容物操作后血浆胃动素浓度的变化。
DOI：
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发表时间：
1981
影响因子：
2.1
通讯作者：
E. Daniel
中科院分区：
文献类型：
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作者：
S. Collins;T. D. Lewis;J. Fox;N. Track;M. Meghji;E. Daniel
在24名健康空腹受试者中研究了各种刺激后免疫反应性血浆胃动素浓度。血浆胃动素通过使用抗体GP 71（J.C.棕色）和天然猪胃动素作为标准品。基础胃动素水平范围从检测不到到365 pg/mL。胃窦和十二指肠内输注50 mL o.1 N HCl（pH 1.2）以5 mL/min的速度输注未能显著改变血浆胃动素水平，但以17 mL/min的速度输注十二指肠酸导致血浆胃动素水平显著升高。（70.8 +/- 29.5 pg/mL，平均值+/- SEM; n = 6），在40 min时达到最大值。以5 mL/min输注50 mL 0.3 M Tris缓冲液（pH 8.0）进行十二指肠碱化，血浆胃动素无变化。混合餐不影响血浆胃动素水平。摄入60 g脂肪显著增加血浆胃动素（n = 13; 30 min时最大增加150.3 +/- 43.3 pg/mL），但十二指肠灌注脂肪未能增加血浆胃动素水平。这些结果表明，由脂肪诱导的胃动素分泌需要脂肪最初存在于胃内，以便分泌发生。我们的结论是，摄入的脂肪是胃动素释放的一个强有力的刺激。由于十二指肠酸化（50毫升0.1 N盐酸超过10分钟）诱导十二指肠活动类似于迁移运动复合体，但不释放胃动素，我们的数据反对释放胃动素后，十二指肠酸化作为触发这些复合体在人。
Immunoreactive plasma motilin concentrations were studied following a variety of stimuli in 24 healthy fasting subjects. Plasma motilin was measured by a radioimmunoassay using antibody GP 71 (J. C. Brown) and natural porcine motilin as standard. Basal motilin levels ranged from undetectable to 365 pg/mL. Antral and intraduodenal infusion of 50 mL o.1 N HCl (pH 1.2) at 5 mL/min failed to alter significantly plasma motilin levels but duodenal acid infusions at 17 mL/min caused a significant increase (70.8 +/- 29.5 pg/mL, mean +/- SEM; n = 6), maximal at 40 min. Duodenal alkalinization with 50 mL 0.3 M Tris buffer (pH 8.0) infused at 5 mL/min produced no change in plasma motilin. A mixed meal did not affect plasma motilin levels. Ingestion of 60 g fat significantly increased plasma motilin (n = 13; maximal increase 150.3 +/- 43.3 pg/mL at 30 min) but duodenal infusions of fat failed to increase plasma motilin levels. These results suggest that motilin secretion induced by fat requires that the fat be present initially within the stomach for secretion to occur. We conclude that ingested fat is a potent stimulus of motilin release. As duodenal acidification (50 mL 0.1 N HCl over 10 min) induces duodenal activity resembling migrating motor complexes but does not release motilin, our data argue against the release of motilin following duodenal acidification as a trigger for the initiation of these complexes in man.