Antigen presentation by dendritic cells after immunization with DNA encoding a major histocompatibility complex class II-restricted viral epitope.
Antigen presentation by dendritic cells after immunization with DNA encoding a major histocompatibility complex class II-restricted viral epitope.
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DNA免疫后，树突状细胞的抗原表现，编码主要的组织相容性复合物II类限制性病毒表位。
DOI：
10.1084/jem.186.9.1481
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发表时间：
1997-11-03
期刊：
影响因子：
--
通讯作者：
Bona CA
中科院分区：
文献类型：
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作者：
Casares S;Inaba K;Brumeanu TD;Steinman RM;Bona CA
用裸DNA进行肌内和皮内免疫可以使动物接种编码的蛋白质，但抗原呈递的潜在机制尚不清楚。我们使用编码A/PR/8/34流感肽的DNA用于CD 4 T细胞，并增强保护性抗病毒免疫。经DNA转染的培养肌肉细胞释放流感多肽，然后可以将其提呈在树突状细胞的主要组织相容性复合体II类分子上。将DNA注射到肌肉或皮肤中，从引流淋巴结或皮肤中分离抗原呈递细胞，树突细胞（而非B细胞）将抗原呈递给T细胞并携带质粒DNA。我们认为，树突状细胞表达的DNA和/或蛋白质的摄入引发了对DNA疫苗的免疫反应。
Intramuscular and intracutaneous immunization with naked DNA can vaccinate animals to the encoded proteins, but the underlying mechanisms of antigen presentation are unclear. We used DNA that encodes an A/PR/8/34 influenza peptide for CD4 T cells and that elicits protective antiviral immunity. DNA-transfected, cultured muscle cells released the influenza polypeptide, which then could be presented on the major histocompatibility complex class II molecules of dendritic cells. When DNA was injected into muscles or skin, and antigen-presenting cells were isolated from either the draining lymph nodes or the skin, dendritic, but not B, cells presented antigen to T cells and carried plasmid DNA. We suggest that the uptake of DNA and/or the protein expressed by dendritic cells triggers immune responses to DNA vaccines.