Suppression of agglomeration of ciprofloxacin-loaded human serum albumin nanoparticles
Suppression of agglomeration of ciprofloxacin-loaded human serum albumin nanoparticles
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DOI：
10.1208/pt0801017
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Jain, Narendr K.
中科院分区：
文献类型：
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作者：
Kumar, P. Vijayaraj;Jain, Narendr K.
本研究旨在开发和探索果胶在抑制载有环丙沙星的人血清白蛋白（HSA）纳米颗粒的聚集方面的用途。采用pH凝聚法制备负载环丙沙星的HSA-果胶纳米粒，并对纳米粒的粒径、形态zeta电位、电解质诱导絮凝、pH依赖性zeta电位、载药量、体外药物释放和稳定性等各种理化参数进行了评价。 HSA-果胶纳米粒子 (F3) 的尺寸为 180 至 290 nm。当纳米颗粒在Na2SO4溶液中的临界絮凝浓度从0.3 M增加到0.9 M时，用果胶对HSA纳米颗粒进行修饰。发现形成的纳米颗粒的等电点在pH值3和6之间相对较低。果胶可以用作抑制HSA纳米颗粒中颗粒团聚的药物添加剂，其效果可能归因于纳米颗粒表面存在的果胶链段。
The present study is aimed at developing and exploring the use of pectin in suppression of agglomeration of ciprofloxacin-loaded human serum albumin (HSA) nanoparticles. The HSA-pectin nanoparticles loaded with ciprofloxacin were prepared by the pH-coacervation-method, and various physicochemical parameters such as particle size, morphology zeta-potential, electrolyte-induced flocculation, pH-dependent zeta-potential, drug loading, in vitro drug release, and stability of nanoparticles, were evaluated. The size of the HSA-pectin nanoparticles (F3) was found to be 180 to 290 nm. The HSA nanoparticles were modified with pectin when the critical flocculation concentration of nanoparticles in Na2SO4 solution was increased from 0.3 M to 0.9 M. The isoelectric points of the formed nanoparticles were found to be relatively lower between pH values 3 and 6. Pectin may be used as a pharmaceutical additive for the suppression of particle agglomeration in HSA nanoparticles, and the effect may be attributed to the pectin segments present on the surface of nanoparticles.